金属有机框架Cu@Sc-MOF纳米酶对5'-三磷酸腺苷的比色/荧光检测  

Colorimetric/Fluorometric Detection of Adenosine-5′-Triphosphate Based on Metal-Organic Framework Cu@Sc-MOF Nanozyme

作　　者：柴小静 赵瑞瑞 张羱 董川 双少敏[1] CHAI Xiao-Jing;ZHAO Rui-Rui;ZHANG Yuan;DONG Chuan;SHUANG Shao-Min(School of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China;Institute of Environmental Science,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学化学化工学院,太原030006 [2]山西大学环境科学研究所,太原030006

出　　处：《应用化学》2024年第5期728-738,共11页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(No.22274090)资助。

摘　　要：通过溶剂热法制备了一种铜/钪金属有机框架(Cu@Sc-MOF)纳米酶,在H2O2存在下,Cu@Sc-MOF可催化氧化3,3’,5,5’-四甲基联苯胺(TMB)得到蓝色氧化产物oxTMB,并在652 nm处产生特征吸收峰。同样,Cu@Sc-MOF也可氧化邻苯二胺(OPD)生成2-氨基吩嗪(DAP),并在570 nm产生特征荧光发射峰(激发波长为390 nm)。由于5’-三磷酸腺苷(ATP)与Cu2+络合,抑制了Cu@Sc-MOF的催化活性,使得652 nm处的吸光度和570 nm处的荧光强度减弱,基于Cu@Sc-MOF的类过氧化物酶活性,构建了一种用于检测ATP的比色/荧光双模式光谱法。比色和荧光分析法的线性范围分别为2.50~40.00μmol/L和1.00~22.50μmol/L,检出限(LOD)分别为0.60和0.27μmol/L。将上述比色/荧光双模式分析法用于肝癌细胞HepG2细胞裂解液中ATP的检测,加标回收率分别为92.0%~101%和93.2%~97.9%,具有良好的应用前景。A copper/scandium metal-organic framework(Cu@Sc-MOF)nanozyme was prepared by solvothermal method.The Cu@Sc-MOF nanozyme could oxidize substrate 3,3′,5,5′-tetramethylbenzidine(TMB)to produce blue oxTMB which showed absorption at 652 nm in the presence of H2O2.Similarly,Cu@Sc-MOF was used to catalyze o-phenylenediamine(OPD)to generate diminophenazine(DAP)with fluorescence emission at 570 nm.The colorimetric/fluorometric dual-mode sensor for the detection of adenosine-5′-triphosphate(ATP)was developed owing to the peroxidase-like characteristics of the [e-mail] the addition of ATP in the Cu@Sc-MOF/H2O2 mixture,the peroxidase-like activity of Cu@Sc-MOF was inhibited due to the strong interaction between ATP and Cu2+ion,causing weakened absorbance at 652 nm and less fluorescence intensity at 570 nm.The respective values of linear ranges for the colorimetric and fluorometric method were 2.50~40.0μmol/L and 1.00~22.5μmol/L,and the limits of detection(LOD)were 0.600 and 1.87μmol/L.The colorimetric/fluorescence dual-mode analysis method could realize to detect ATP in HepG2 cell lysate with recoveries ranged from 92.0%~101%and 93.2%~97.9%,respectively,showing good application prospects in the detection of ATP.

关 键 词：Cu@Sc-MOF 纳米酶 类过氧化物酶活性 比色/荧光 5’-三磷酸腺苷 

分 类 号：O655[理学—分析化学]