Detection of ribophagy in yeast and mammals  

作　　者：Miao Ye Yuting Chen Zhaojie Liu Yigang Wang Cong Yi 

机构地区：[1]Xinyuan Institute of Medicine and Biotechnology,School of Life Sciences and Medicine,Zhejiang Sci-Tech University,Hangzhou 310018,China [2]Department of Biochemistry,and Department of Hepatobiliary and Pancreatic Surgery of the First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310058,China [3]Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation,Affiliated Cancer Hospital&Institute of Guangzhou Medical University,School of Basic Medical Sciences,Guangzhou Medical University,Guangzhou 510182,China

出　　处：《Biophysics Reports》2024年第2期82-101,共20页生物物理学报（英文版）

摘　　要：Ribophagy,the cellular process wherein ribosomes are selectively self-digested through autophagy,plays a pivotal role in maintaining ribosome turnover.Understanding the molecular regulatory mechanisms governing ribophagy is pivotal to uncover its significance.Consequently,the establishment of methods for detecting ribophagy becomes important.In this protocol,we have optimized,enriched,and advanced existing ribophagy detection techniques,including immunoblotting,fluorescence microscopy,and transmission electron microscopy(TEM),to precisely monitor and quantify ribophagic events.Particularly noteworthy is the introduction of TEM technology for yeast ribophagy detection.In summary,the delineated methods are applicable for detecting ribophagy in both yeast and mammals,laying a solid foundation for further exploring the physiological importance of ribophagy and its potential implications in diverse cellular environments.

关 键 词：Ribophagy Ribosome turnover Immunoblotting Fluorescence microscopy Transmission electron microscopy Yeast and mammals 

分 类 号：Q25[生物学—细胞生物学]