干细胞外泌体对肺型氧中毒的保护作用和机制  

Protective effect and mechanism of mesenchymal stem cell derived exosomes against pulmonary oxygen toxicity

作　　者：徐胜 崔立昆 王舒 陈杰 包晓辰 王越 赵云鹏 XU Sheng;CUI Likun;WANG Shu;CHEN Jie;BAO Xiaochen;WANG Yue;ZHAO Yunpeng(Department of Stem Cell and Regeneration Medicine,Translational Medicine Research Center,Naval Medical University,Shanghai 200433,China;National Key Laboratory of Immunity&Inflammation,Naval Medical University,Shanghai 200433,China;Shanghai Institute of Stem Cell Research and Clinical Translation,Shanghai 200092,China;Department of Diving Medicine,Naval Medical Center,Shanghai 200433,China;Shanghai Key Laboratory of Cell Engineering,Shanghai 200433,China)

机构地区：[1]海军军医大学转化医学中心干细胞与再生医学研究室,上海200433 [2]海军军医大学免疫与炎症全国重点实验室,上海200433 [3]上海市干细胞临床转化研究院,上海200092 [4]海军特色医学中心潜水教研室,上海200433 [5]上海市细胞工程重点实验室,上海200433

出　　处：《军事医学》2024年第4期267-272,共6页Military Medical Sciences

基　　金：国家自然科学基金(82071789);上海市Ⅳ类高峰学科建设基金项目。

摘　　要：目的探讨干细胞外泌体在肺型氧中毒中的保护作用及机制。方法实验小鼠分为常压空气对照组、高压氧暴露组和高压氧外泌体干预组。在暴露前1 d给予磷酸盐缓冲液(PBS)和外泌体处理,0.23 MPa纯氧暴露8 h,分析肺部湿干比、炎症、渗出情况,病理切片分析肺损伤,并检测肺部细胞死亡、抗氧化相关分子表达。结果干细胞外泌体预处理可明显减轻高压氧暴露引起的肺部损伤,降低湿干比,减少炎症因子分泌和肺部细胞凋亡。结论干细胞外泌体预防性给药可通过减少细胞死亡和炎症,减轻高压氧引起的肺型氧中毒。Objective To investigate the role and mechanism of exosomes derived from mesenchymal stem cells in hyperbaric oxygen caused pulmonary oxygen toxicity.Methods Mice were divided into the control group that was exposed to normal air,and hyperbaric oxygen exposure groups treated with phosphate buffer saline(PBS)or exosomes,respectively.PBS and exosome treatment were given one day prior to exposure.Mice were subjected to 0.23 MPa pure oxygen for 8 hours.The lung wet-dry ratio,inflammation,exudation and pathological injury were analyzed,while cell death and antioxidant related molecules were detected.Results Pretreatment with exosome significantly attenuated lung injury caused by hyperbaric oxygen exposure by decreasing the lung wet-dry ratio,inflammation,and cell apoptosis.Conclusion Prophylactic administration of exosomes derived from mesenchymal stem cells can alleviate hyperbaric oxygen induced pulmonary oxygen poisoning by attenuating cell death and inflammation.

关 键 词：外泌体 肺型氧中毒 肺损伤 

分 类 号：Q813[生物学—生物工程] R835[医药卫生—航空、航天与航海医学]