小鼠外周血单个核细胞对囊胚的免疫应答反应探究  

Immunological Behavior of Peripheral Blood Mononuclear Cells on Mouse Blastocyst

作　　者：滕亚迪 刘子涵 马梅香 安礼友 Teng Yadi;Liu Zihan;Ma Meixiang;An Liyou(School of Life Science and Technology,Xinjiang University,Urumqi 830017,China;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,Xinjiang University,Urumqi 830017,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830017 [2]新疆大学新疆生物资源基因工程重点实验室,新疆乌鲁木齐830017

出　　处：《南京师范大学学报（工程技术版）》2024年第2期59-66,共8页Journal of Nanjing Normal University(Engineering and Technology Edition)

基　　金：新疆维吾尔自治区自然科学基金杰出青年科学基金项目(2022D01E09)。

摘　　要：精卵结合形成受精卵,胚胎经过发育形成囊胚,囊胚孵化后与子宫互作引发着床.已有研究显示胚胎着床是一个炎性过程,基因上的异质性决定了胚胎会引发子宫的免疫反应.但是,目前对胚胎的免疫特性的了解还很少.为解析胚胎与免疫细胞可能发生的免疫互作,本研究以外周血单个核细胞(PBMC)为细胞模型,评价小鼠囊胚对白细胞的免疫学作用.分别将10、20枚小鼠囊胚与PBMC共培养24 h,同时以脂多糖(LPS)处理为对照.研究结果显示,胚胎可诱导PBMC增殖,并显著降低T细胞亚群比例(P<0.05);LPS处理PBMC引发先天免疫细胞NK、单核细胞增殖,胚胎的加入进一步增加了NK、单核细胞增殖(P<0.05).对培养液中的细胞因子检测发现,PBMC与胚胎共培养后,促炎性分子IL-6和抑炎分子IL-10均显著增加(P<0.05);值得注意的是胚胎能下调LPS诱导的TNF-α表达(P<0.05).结果表明,囊胚对PBMC亚群具有调控作用,能增强LPS引发的先天免疫反应,可以调控细胞因子IL-6、TNF-α和IL-10的分泌.研究结果提示小鼠囊胚可能不仅能引起子宫内膜发生炎性反应,而且能对子宫免疫细胞功能进行动态调节.研究为进一步解析着床过程中的母胎免疫识别提供了数据参考.The egg is fertilized a sperm forming a zygote,and then develops to form a blastocyst with series developmental events.Blastocyst hatches out of ZP and interacts with the uterus initiating implantation.It has been shown that embryo implantation is an inflammatory process.The genetic heterogeneity of the embryo determines that the embryo will trigger an immune response in the uterus.However,the immunological properties of the embryo have yet to be resolved.To investigate the possible immune interaction between embryos and immune cells,we use peripheral blood mononuclear cells(PBMC)to evaluate the immunological effects of mouse blastocysts.10 and 20 mouse blastocysts are co-cultured with PBMC for 24 hours,and lipopolysaccharide(LPS)treatment is used as a control respectively.Results show that embryos induce PBMC proliferation,in which the proportion of T cells is reduced significantly(P<0.05).When treated by LPS,NK cells and monocytes which are innate immune cells,PBMC are induced in proliferation.Treating both LPS and embryos,NK cells and monocytes are further proliferated(P<0.05).The cytokines in medium is analyzed by ELISA.Data show that the pro-inflammatory molecule IL-6 and the anti-inflammatory molecule IL-10 are significantly increased after PBMC are cultivated with embryos(P<0.05).Interestingly,embryos can down-regulate the expression of LPS-induced TNF-α(P<0.05).Results show that blastocysts have a regulatory effect on PBMC subpopulation,enhance LPS-triggered innate immune response,and regulate the secretion of cytokines IL-6,TNF-αand IL-10.Our findings imply that mouse blastocysts do not only cause an inflammatory response in the endometrium,but also have a dynamic regulatory effect on uterine immune cells.This study provides insights for further analysis of uterus-embryo immunological interaction during implantation.

关 键 词：囊胚 单个核细胞 共培养 免疫应答 细胞因子 

分 类 号：Q813.7[生物学—生物工程]