同期发情处理后荷斯坦奶牛外周血生殖激素水平变化  

Changes of reproductive hormone levels in peripheral blood of Holstein dairy cows after estrus synchronization

作　　者：王中禹 郭佳丽 蒋超祥 罗永明[2,4] 郑新宝 WANG Zhongyu;GUO Jiali;JIANG Chaoxiang;LUO Yongming;ZHENG Xinbao(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Animal Science,Xinjiang Academy of Animal Husbandry,Urumqi 830011,China;Xinjiang Bayingoleng Mongolian Autonomous Prefecture Animal Husbandry Workstation,Bayingoleng 841000,China;Key Laboratory of Evaluation and Utilization of Livestock and Poultry Resources(Sheep),Ministry of Agriculture and Rural Affairs,Urumqi 830026,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]新疆畜牧科学院畜牧研究所,乌鲁木齐830011 [3]新疆巴音郭楞蒙古自治州畜牧工作站,新疆巴音郭楞841000 [4]农业农村部畜禽资源(羊)评价利用重点实验室,乌鲁木齐830026

出　　处：《黑龙江动物繁殖》2024年第3期1-7,共7页Heilongjiang journal of animal reproduction

基　　金：新疆维吾尔自治区重大专项“牛高效繁殖技术研究”(2023A02011-1);新疆维吾尔自治区奶产业技术体系项目(XJARS-11)。

摘　　要：为了提高奶牛同期发情-定时输精技术的受胎率,试验研究同期发情处理后荷斯坦奶牛外周血中生殖激素含量的变化,探讨荷斯坦奶牛的人工授精最佳时间。选择24头空怀青年荷斯坦奶牛,使用抑制素(INH)结合同期发情-定时输精(TAI)程序处理,分别在第一次肌肉注射抑制素抗原和布舍瑞林前(记作第0天)、第一次肌肉注射PG前(记作第7天)、第二次注射布舍瑞林前(记作第9天),以及第一次人工输精前(记作第10天)采集外周血,采用ELISA法检测外周血中抗缪氏管激素(AMH)、INH(以抑制素B即INHB为代表)、促卵泡素(FSH)和促黄体素(LH)的浓度,分析母牛在同期发情处理前后外周血中生殖激素水平的变化、生殖激素浓度的相关性和各个阶段的差异性。结果表明:第7天试验牛外周血中LH含量极显著高于第0天、第9天以及第10天(P<0.01),并且第9天试验牛外周血中LH含量显著高于第10天(P<0.05);第9天试验牛外周血中FSH含量极显著高于第0天和第10天(P<0.01),并且第7天试验牛外周血中FSH含量极显著高于第10天(P<0.01);而AMH、INHB含量没有显著变化(P>0.05),且不同个体之间不同激素含量的变化波动明显减小,达到相对一致水平。说明INHB、AMH在调控卵泡期FSH的分泌过程中发挥了重要的作用,促进了卵泡的发育;在奶牛同期发情-定时输精过程中,可以通过监测奶牛体内的INHB、AMH、FSH、LH等生殖激素水平,精确判断排卵和人工授精时间,从而提高奶牛同期发情-定时输精效果。In order to improve the conception rate of estrus synchronization-timed insemination technology in cows,this experiment studied the changes of reproductive hormone levels in the peripheral blood of Holstein cows after estrus synchronization and explored the optimal time for artificial insemination in Holstein cows.Twenty-four young barren Holstein cows were chosen for the experiment and treated with a combination of inhibin and estrus synchronization and timed insemination(TAI)program.Peripheral blood was collected before the first intramuscular injection of inhibin antigen and Buserelin(day 0),the first intramuscular injection of PG(day 7),the second injection of Buserelin(day 9),and the first artificial insemination(day 10).ELISA was used to detect the concentrations of AMH,inhibin(INH,represented by INHB),follicle stimulating hormone(FSH),and luteinizing hormone(LH)in peripheral blood of cows.The changes in reproductive hormone levels in peripheral blood of cows before and after estrus synchronization treatment,as well as the correlation and differences in reproductive hormone concentrations at different experimental stages,were analyzed.The results showed that the LH content at day 7 was extremly higher than at day 0,day 9 and day 10(P<0.01),among which,the LH content at day 9 was significantly higher than at day 10(P<0.05)in the peripheral blood of cows.The FSH content at day 9 was extremly higher than at day 0 and day 10(P<0.01),the FSH content at day 7 was extremly higher than at day 10(P<0.01)in the peripheral blood of cows.However,no significant change in AMH and INHB contents(P>0.05)and the fluctuation of different hormone contents among different individuals was found,showing a relatively consistent level.The results of this experiment indicated that both INHB and AMH could play important roles in regulating the secretion of FSH during the follicular phase and promoting follicular development.

关 键 词：同期发情 荷斯坦奶牛 外周血 生殖激素 人工授精 

分 类 号：S823.3[农业科学—畜牧学]