IFN-β通过STAT1诱导SARI表达抑制AML细胞增殖并促进凋亡  

IFN-βinhibits proliferation and promotes apoptosis of AML cells by STAT1-induced SARI

作　　者：林艳凤[1] 洪小颖 黄莹莹 王小花 吴玮 林东红[1] 薛龑[1] LIN Yanfeng;HONG Xiaoying;HUANG Yingying;WANG Xiaohua;WU Wei;LIN Donghong;XUE Yan(The School of Medical Technology and Engineering,Fujian Medical University,Fuzhou 350004,China)

机构地区：[1]福建医科大学医学技术与工程学院,福州350004

出　　处：《中国免疫学杂志》2024年第6期1137-1141,共5页Chinese Journal of Immunology

基　　金：福建省自然科学基金面上项目(2021J01812);福建省中青年教师教育科研项目(科技类)(JAT190178,JAT190179)。

摘　　要：目的:探讨IFN-β诱导SARI表达对急性粒细胞性白血病(AML)细胞增殖、凋亡的作用,并筛选其潜在的调控分子。方法:qPCR、Western blot筛选SARI低表达的AML细胞作为实验细胞株;不同浓度IFN-β干预AML细胞,于不同时间采用qPCR、Western blot检测SARI表达,选取IFN-β作用的适当浓度和时间;采用RNA-Seq转录组测序及KEGG富集分析初步筛选IFN-β诱导AML细胞SARI表达的潜在调控分子;通过相应分子抑制剂联合IFN-β处理AML细胞,MTS法检测细胞增殖,流式细胞术检测细胞凋亡;明确该分子参与IFN-β诱导SARI表达对AML细胞增殖及凋亡的作用。结果:HL60和NB4细胞SARI表达相对较低,选为实验细胞株;1 ng/ml IFN-β作用12 h后AML细胞SARI表达升高且细胞增殖被抑制,凋亡增多;筛选STAT1为IFN-β诱导SARI表达的潜在调控分子;抑制STAT1后,IFN-β对AML细胞SARI表达、增殖抑制、凋亡促进的作用被明显逆转。结论:IFN-β可通过STAT1诱导AML细胞SARI表达,抑制细胞增殖,促进细胞凋亡。Objective:To investigate effect of SARI expression induced by IFN-βon proliferation and apoptosis of acute myelo-blastic leukemia(AML)cells,and to explore its potential regulatory molecules.Methods:qPCR and Western blot were used to screen AML cells with low SARI expression as experimental cell lines.AML cells were treated with different concentrations of IFN-β,and expression of SARI was detected by qPCR and Western blot at different time to select appropriate concentration and time of IFN-β.RNA-Seq transcriptome sequencing and KEGG enrichment analysis were used to preliminarily screen potential regulatory molecules of IFN-β-induced SARI expression in AML cells.AML cells were treated with corresponding molecular inhibitors combined with IFN-β,cell proliferation was detected by MTS assay,and apoptosis was detected by flow cytometry.To clear this molecule was involved in IFN-β-induced SARI expression on AML cell proliferation and apoptosis.Results:SARI expression in HL60 and NB4 cells were rela-tively decreased,so they were selected as experimental cell lines.After treatment with 1 ng/ml IFN-βfor 12 h,SARI expression in AML cells was increased,cell proliferation was inhibited and apoptosis were increased.STAT1 was screened as a potential regulatory mole-cule for IFN-β-induced SARI expression.After inhibiting STAT1,effects of IFN-βon SARI expression,proliferation inhibition and apop-tosis promotion of AML cells were reversed significantly.Conclusion:IFN-βcan promote SARI expression in AML cells by STAT1,in-hibit cell proliferation and promote apoptosis.

关 键 词：IFN-Β SARI STAT1 AML 增殖 凋亡 

分 类 号：R446.1[医药卫生—诊断学]