lncRNA SNHG16通过miR-182-5p/PPARG轴调控类风湿关节炎成纤维样滑膜细胞增殖和侵袭  

lncRNA SNHG16 regulates proliferation and invasion of rheumatoid arthritis fibroblast-like synoviocytes through miR-182-5p/PPARG axis

作　　者：周文煜 陈文莉 甘文渊 申江曼 ZHOU Wenyu;CHEN Wenli;GAN Wenyuan;SHEN Jiangman(Wuhan No.4 Hospital,Wuhan 430030,China;Wuhan Central Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430000,China)

机构地区：[1]武汉市第四医院,武汉430030 [2]华中科技大学同济医学院武汉市中心医院,武汉430000

出　　处：《中国免疫学杂志》2024年第6期1147-1152,共6页Chinese Journal of Immunology

基　　金：武汉市卫生和计划生育委员会科研项目(WZ18Q13)。

摘　　要：目的:确定小核仁RNA宿主基因16(SNHG16)在类风湿关节炎(RA)患者成纤维样滑膜细胞(FLS)中的表达及其在RA发展中的作用。方法:RT-qPCR用于测量SNHG16、miR-182-5p和过氧化物酶体增殖物激活受体-γ(PPARG) mRNA表达;双荧光素酶实验用于检测SNHG16、miR-182-5p和PPARG mRNA的相互作用关系;EdU和Transwell用于检测细胞增殖、侵袭;Western blot用于检测PPARG蛋白水平。结果:RA滑膜组织和人RA-FLS系(HFLS-RA)中SNHG16和PPARG mRNA表达上调,miR-182-5p表达下调。SNHG16负靶向调节miR-182-5p表达,正调节PPARG(miR-182-5p靶标)表达。沉默SNHG16抑制HFLS-RA增殖、侵袭;下调miR-182-5p部分逆转SNHG16沉默对细胞增殖、侵袭的抑制作用;过表达PPARG部分逆转miR-182-5p上调对HFLS-RA增殖、侵袭的抑制作用。结论:沉默SNHG16靶向miR-182-5p/PPARG轴抑制HFLS-RA的增殖、侵袭。Objective:To determine the expression of small nucleolar RNA host gene 16(SNHG16)in fibroblast-like synovio⁃cytes(FLS)of patients with rheumatoid arthritis(RA)and its role in the development of RA.Methods:RT-qPCR was performed to measure SNHG16,miR-182-5p and peroxisome proliferator-activated receptor gamma(PPARG)mRNA expression;dual-luciferase assay was performed to measure the interaction between SNHG16,miR-182-5p and PPARG mRNA;EdU and Transwell were per⁃formed to measure cell proliferation and invasion;Western blot was performed to measure PPARG protein level.Results:SNHG16 and PPARG mRNA expression were up-regulated and miR-182-5p expression was down-regulated in RA synovial tissue and human RA-FLS line(HFLS-RA).SNHG16 negatively targeted miR-182-5p expression and positively regulated PPARG(miR-182-5p target)expression.Silencing of SNHG16 inhibited the proliferation and invasion of HFLS-RA;down-regulation of miR-182-5p partially re⁃versed the inhibitory effect of SNHG16 silencing on cell proliferation and invasion;overexpression of PPARG partially reversed the in⁃hibitory effect of up-regulation of miR-182-5p on the proliferation and invasion of HFLS-RA.Conclusion:Silencing SNHG16 target⁃ing miR-182-5p/PPARG axis inhibits the proliferation and invasion of HFLS-RA.

关 键 词：类风湿关节炎 成纤维样滑膜细胞 lncRNA SNHG16 miR-182-5p PPARG 

分 类 号：R593.22[医药卫生—内科学]