基于Box-Behnken响应面法优化鹿角蛋白的提取工艺  

Optimization of extraction process for deer keratin based on Box-Behnken response surface methodology

作　　者：高建辉 李旭欣 范元赫 张舜华 叶恒 张宇[3] 赵龙山[2] 关雪峰 无 GAO Jianhui;LI Xuxin;FAN Yuanhe;ZHANG Shunhua;YE Heng;ZHANG Yu;ZHAO Longshan;GUAN Xuefeng(School of Life Sciences,Shenyang Pharmaceutical University,Shenyang 110016,China;School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

机构地区：[1]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]辽宁中医药大学,辽宁沈阳110032 [4]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《沈阳药科大学学报》2024年第6期742-747,774,共7页Journal of Shenyang Pharmaceutical University

基　　金：中央引导地方科技发展资金(自由探索类基础研究)资助项目(2023JH6/100100007)。

摘　　要：目的 通过Box-Behnken响应面法优化鹿角蛋白的提取工艺。方法 在单因素考察基础上进行响应面法设计,寻找最佳提取条件。以鹿角中蛋白含量为考察指标,使用考马斯亮蓝染色法测量样品溶液吸光度,再通过回归方程计算出样品溶液的鹿角蛋白含量。结果 最优提取工艺条件为NaOH浓度0.35 mol·L^(-1),液料比6:1,提取时间114 min。结论 经验证通过Box-Behnken响应面法所得到的预测值与测定的实际值偏差均在±1%以内,可以为膝痛康中鹿角蛋白的提取工艺提供参考。Objective To optimize the extraction process of antler protein by Box-Behnken response surface method.Methods The response surface method was designed on the basis of univariate investigation to find the best extraction conditions.The protein content in the antler was used as the investigation index,the absorbance of the sample solution was determined,and the protein content of the sample was calculated according to the standard curve equation.Results The optimal extraction process conditions were as follows:NaOH concentration was 0.35 mol·L^(-1),liquid-to-material ratio was 6:1,extraction time was 114 min.Conclusion It is verified that the deviation between the actual measured value and the predicted value obtained by the Box-Behnken response surface method is within±1%,and the detection results are good,which can provide a reference for the extraction process of antler protein in Xitongkang.

关 键 词：鹿角 Box-Behnken响应面 提取工艺 蛋白含量 

分 类 号：R932[医药卫生—生药学]