云南省边境地区鼠疫基因组流行病学研究  

Molecular epidemiological analysis of plague at the border area of Yunnan Province

作　　者：杨丰义 杨荣 李思儒 孔进姣 谭红丽[2] 张海鹏[2] 王鹏[2] 钟佑宏[2] 石丽媛[2] 宋志忠[3] YANG Feng-yi;YANG Rong;LI Si-ru;KONG Jin-jiao;TAN Hong-li;ZHANG Hai-peng;WANG Peng;ZHONG You-hong;SHI Li-yuan;SONG Zhi-zhong(School of Public Health,Dali University,Dali 671000,China;Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention,Yunnan Institute for Endemic Disease Control and Prevention,Dali 671000,China;Yunnan Center for Disease Control and Prevention,Kunming 650022,China)

机构地区：[1]大理大学公共卫生学院,大理671000 [2]云南省地方病防治所云南省自然疫源性疾病防控技术重点实验室,大理671000 [3]云南省疾病预防控制中心,昆明650022

出　　处：《中国人兽共患病学报》2024年第5期401-407,共7页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.82260659);云南省高层次卫生健康技术人才培养项目(No.L-2019001,No.D-2019002,No.H-2019003);云南省地方病防治所科技计划项目(No.YIEDC-T202105)联合资助。

摘　　要：目的探索云南边境地区鼠疫的流行病学特征及所分离鼠疫菌株的遗传进化关系,为进一步研究1982-2007年的第2次流行的起因及流行规律提供线索。方法收集1982-2007年鼠疫流行期间边境地区的鼠疫疫情资料进行描述性流行病学分析;并获得262株边境地区鼠疫菌株的全基因组序列,进行系统发育分析。结果云南省有17个边境县(市)发生鼠疫疫情,判定疫点数552个,病例数123例。云南边境地区分离鼠疫菌中存在1.ORI2、1.ORI3、1.IN3、2.ANT和2.MED 5种基因型,其中1.ORI2为优势种群。258株1.ORI2种群的云南鼠疫菌分属于4个亚簇。在整个系统发育关系中,缅甸及越南支系嵌入在云南支系内。结论1982-2007年云南省边境地区鼠疫流行强度大,呈现由西向南、向东发展的趋势;云南与毗邻国家存在鼠疫跨境传播的风险,应加强边境地区鼠疫监测及防控工作。This study was aimed at exploring the epidemiological characteristics of plague,and the evolutionary relationships among the isolated plague strains in the Yunnan border area,to provide clues for further studying epidemic causes and epidemiological patterns.Plague epidemic data in the border area during the second epidemic period(1982-2007) were collected and analyzed with descriptive epidemiological methods.Whole genome sequences of 262 strains of Yersinia pestis in the border area were obtained for phylogenetic analysis.Plague outbreaks occurred in 17 counties(cities) among 25 border counties(cities);a total of 552 epidemic foci and 123 human cases were identified.The 1.ORI2,1.ORI3,1.IN3,2.ANT and 2.MED genotypes were identified among Yersinia pestis isolated from the Yunnan border area,among which the 1.ORI2 population was dominant.A total of 258 strains of Yersinia pestis from the 1.ORI2 population belonged to four subclusters.The Myanmar and Vietnam clade was embedded within the Yunnan clade in the overall phylogeny.The above results indicated that during the second period of the epidemic,the intensity of plague epidemics in Yunnan's border areas was high,showing a trend of development from west to south and east.Our findings indicated a risk of cross-border transmission of plague between Yunnan and neighboring countries;therefore,the surveillance,prevention,and control of plague in border areas should be strengthened.

关 键 词：边境 家鼠鼠疫疫源地 鼠疫菌 基因组流行病学 

分 类 号：R383.3[医药卫生—医学寄生虫学]