广州市环境污水中沙门菌耐药性及携带耐药基因分析  

Antimicrobial resistance and resistance gene carriage by Salmonella bacteria in environmental sewage in Guangzhou

作　　者：苏碧慧 杜广泓 伍小英[1] 何刚[1] 袁俊[3] 谢朝军 SU Bi-hui;DU Guang-hong;WU Xiao-ying;HE Gang;YUAN Jun;XIE Chao-jun(Guangzhou Chest Hospital,Guangzhou 510095,China;School of Public Health,Guangzhou Medical University,Guangzhou 511436,China;Guangzhou Center for Disease Control and Prevention,Guangzhou 510080,China;Guangzhou Huadu District Center for Disease Control and Prevention,Guangzhou 510803,China)

机构地区：[1]广州市胸科医院,广州510095 [2]广州医科大学公共卫生学院,广州511436 [3]广州市疾病预防控制中心,广州510080 [4]广州市花都区疾病预防控制中心,广州510803

出　　处：《中国人兽共患病学报》2024年第5期442-447,共6页Chinese Journal of Zoonoses

基　　金：广东省自然科学基金-面上项目(No.2021A1515012539);广州市科技计划项目(No.202102080295);广州市医学重点学科(No.2021-2023-11);广州市花都医疗卫生一般科研专项项目(No.23-HDWS-079)。

摘　　要：目的 探讨广州市环境污水中沙门菌的耐药性及耐药基因携带情况。方法 收集广州市2022年3月1日至2022年11月30日环境污水中分离培养的140株沙门菌作为研究对象,开展药物敏感性检测及全基因组测序,通过CARD耐药数据库分析全基因组测序结果,获取相应的耐药基因。结果 试验菌株对氨苄西林、四环素、链霉素、氯霉素、复方新诺明的耐药率较高,均达80%以上。多粘菌素E、环丙沙星的中介率较高,达60%以上。多重耐药现象严重,多重耐药率高达92.86%。菌株携带多种类型的耐药基因,特别是氨基糖苷类,携带率高达92.68%。结论 广州市环境污水中沙门菌的耐药以及多重耐药情况严重,多重耐药率整体呈逐渐上升的时间趋势,耐药谱具有多样性,耐药基因等可移动遗传元件介导的耐药机制为耐药和多重耐药产生的主要原因。This study explored the antibiotic resistance and resistance gene carriage of 140 Salmonella strains isolated from environmental sewage in Guangzhou city between March 1,2022,and November 30,2022.The micro broth dilution method was used to select 17 antibiotics for susceptibility testing.According to whole genome sequencing results,the CARD resistance database was used to obtain corresponding resistance genes.High antimicrobial resistance rates above 80% were observed against ampicillin,tetracycline,streptomycin,chloramphenicol,and cotrimoxazole.The intermediation rate of polymyxin E and ciprofloxacin exceeded 60%.The multiple drug resistance status was severe,and the rate of multiple drug resistance was as high as 92.86%.The strains carried multiple types of resistance genes,particularly for aminoglycosides,with a carriage rate as high as 92.68%.The resistance of Salmonella in environmental wastewater in Guangzhou to one or more drugs was severe,and the overall multi-drug resistance rate gradually increased over time.The resistance spectrum was diverse,and the resistance mechanism,mediated by mobile genetic elements such as resistance genes,was found to be the main cause of resistance to one or more drugs.

关 键 词：环境污水 沙门菌 耐药性 耐药基因 

分 类 号：R383.3[医药卫生—医学寄生虫学]