雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究  

Induction of apoptosis in triple-negative breast cancer cells by petroleum ether extract of Sageretia thea

作　　者：冯志惠 邓仪卿 叶冰 安培 张宏 张海军[1] FENG Zhihui;DENG Yiqing;YE Bing;AN Pei;ZHANG Hong;ZHANG Haijun(Innovation Drug Research Center,School of Life Sciences,Huaibei Normal University,Huaibei 235000,China;Institute of Interdisciplinary Integrative Medicine Research,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;TCM Outpatient Department of Yunnan Hongrentang TCM Hospital Co.,Ltd,Kunming 650500,China)

机构地区：[1]淮北师范大学生命科学学院创新药物研究中心,安徽淮北235000 [2]上海中医药大学交叉科学研究院,上海201203 [3]云南鸿仁堂健康管理有限公司呈贡中医门诊部,云南昆明650500

出　　处：《药学实践与服务》2024年第6期253-259,共7页Journal of Pharmaceutical Practice and Service

基　　金：安徽省高等学校科学研究项目(2023AH040055);安徽省自然科学基金项目(2308085MC79);安徽省高校优秀创新团队(2022AH010029)。

摘　　要：目的探讨雀梅藤石油醚提取物对乳腺癌细胞增殖和凋亡的影响。方法石油醚提取物孵育乳腺癌细胞不同时间后,CCK8法检测细胞活力,平板克隆形成法检测细胞增殖,DAPI染色法观察细胞核形态变化,免疫荧光检测线粒体膜电位和活性氧变化,流式细胞术检测细胞周期和凋亡,CCK8法观察对人正常血管内皮细胞毒性。结果雀梅藤石油醚提取物孵育BT549和MDA-MB-231人三阴性乳腺癌细胞株24 h后的IC_(50)分别为45.40μg/ml和12.23μg/ml,时间和剂量依赖地抑制乳腺癌细胞活力和克隆形成,诱导细胞凋亡,阻滞细胞周期于G1/S期,降低线粒体膜电位,升高活性氧;对人正常血管内皮细胞无明显毒性作用。结论雀梅藤石油醚提取物可能通过升高活性氧,引起线粒体膜电位坍塌,激活线粒体通路诱导细胞凋亡,从而抑制乳腺癌细胞生长,支持其民间抗乳腺肿瘤的应用。Objective To investigate the effect of the petroleum ether extract of Sageretia thea on the proliferation and apoptosis of breast cancer cells.Methods After breast cancer cells were incubated with the petroleum ether extract for different times,cell viability was analyzed by CCK8 assay,cell proliferation was detected by plate cloning test,nuclear morphology was observed by DAPI staining,mitochondrial membrane potential(MMP)and reactive oxygen species(ROS)were determined by immunofluorescence,and the cell cycle and apoptosis were detected by flow cytometry.After incubating with the extract for 24 h,the CCK8 assay was used to observe the toxicity to normal human vascular endothelial cells.Results The IC_(50) of BT549 and MDA-MB-231 breast cancer cell lines treated with the petroleum ether extract for 24 h were 45.40μg/ml and 12.23μg/ml,respectively.The extract time and dose dependently inhibited breast cancer cell viability and clonal formation,induced cell apoptosis and cycle arrest in G1/S phase,decreased MMP and increased ROS levels.There was no toxic effect on normal endothelial cells.Conclusion The petroleum ether extract of Sageretia thea may induce apoptosis by increasing ROS to cause MMP collapse,followed by activating mitochondrial pathway,thereby hindering the growth of breast cancer cells.These results could support the application of Sageretia thea to anti-breast tumor in the folk.

关 键 词：雀梅藤 乳腺癌 增殖 凋亡 线粒体 活性氧 

分 类 号：R285[医药卫生—中药学]