基于LC-MS/MS法的芍药苷在正常和肝内胆汁淤积大鼠体内的药代动力学比较研究  

Pharmacokinetics of paeoniflorin in normal and intrahepatic cholestatic rats by LC-MS/MS

作　　者：杨梦换 蒋罡 陈卫东 张善堂 王汝琳[1,2] YANG Meng-huan;JIANG Gang;CHEN Wei-dong;ZHANG Shan-tang;WANG Ru-lin(College of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012;Miinistry of Education-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials,Hefei 230012;Department of Pharmacy,The First Affiliated Hospital of USTC,Anhui Provincial Hospital,Hefei 230001)

机构地区：[1]安徽中医药大学药学院,合肥230012 [2]省部共建安徽道地中药材品质提升协同创新中心,合肥230012 [3]安徽省立医院药剂科,合肥230001

出　　处：《中南药学》2024年第6期1418-1422,共5页Central South Pharmacy

基　　金：国家自然科学基金青年科学基金项目(No.82104517)。

摘　　要：目的比较正常和17α-乙炔基雌二醇诱导的胆汁淤积性肝损伤大鼠体内芍药苷的药代动力学过程的差异。方法采用LC-MS/MS法检测给药后不同时间点大鼠血浆样本中芍药苷的含量,DAS 2.0软件测算两组的药代动力学参数,并进行统计学分析。结果与正常大鼠相比,胆汁淤积性肝损伤发生后,芍药苷的t_(1/2Z)、MRT_(0~∞)、AUC和V_(z)/F明显增加,而CL_(z)/F显著降低。结论与正常大鼠相比,胆汁淤积模型大鼠体内芍药苷的浓度更高、消除速度更缓慢。Objective To compare the pharmacokinetic process of paeoniflorin in normal rats and rats with 17α-ethinylestradiol-induced cholestatic liver injury.Methods The content of paeoniflorin in the rat plasma samples at different time points after the administration was detected by LC-MS/MS.The pharmacokinetic parameters of the two groups were analyzed by DAS 2.0 software.Results The pharmacokinetic behaviors of rats changed after the development of cholestatic liver injury as compared to those of normal rats.Specifically,t_(1/2Z),MRT_(0~∞),AUC and V_(z)/F of paeoniflorin were obviously increased,while CL_(z)/F was greatly decreased.Conclusion Compared with the normal rats,the concentration of paeoniflorin is higher and the elimination rate slower in the cholestatic model rats.

关 键 词：芍药苷 17Α-乙炔基雌二醇 胆汁淤积性肝损伤 药代动力学 

分 类 号：R284.1[医药卫生—中药学] R932.5[医药卫生—中医学]