羟基积雪草苷通过抑制NF-κB/NLRP3细胞焦亡通路缓解间质性膀胱炎  

Madecassoside alleviates interstitial cystitis by inhibiting the pyroptosis of NF-κB/NLRP3 pathway

作　　者：李琪 浦东 王思思 谢璇 李霁 于锋 LI Qi;PU Dong;WANG Si-si;XIE Xuan;LI Ji;YU Feng(School of Basic Medicine and Clinical Pharmacy,China Pharmaceutical University,Nanjing 211198)

机构地区：[1]中国药科大学基础医学与临床药学学院,南京211198

出　　处：《中南药学》2024年第6期1571-1576,共6页Central South Pharmacy

摘　　要：目的 通过NF-κB/NLRP3介导的细胞焦亡途径探究羟基积雪草苷对间质性膀胱炎的相关作用。方法 通过脂多糖建立体外间质性膀胱炎细胞模型,使用不同浓度的羟基积雪草苷处理,采用MTT、单层细胞划痕伤口实验、实时荧光定量PCR和蛋白印迹实验检测羟基积雪草苷对SV-HUC-1细胞间质性膀胱炎体外模型中细胞活力,伤口愈合能力和NF-κB/NLRP3通路介导的细胞焦亡相关蛋白和mRNA的表达的作用。结果 体外细胞实验结果显示,与模型组相比,羟基积雪草苷能改善细胞活力和伤口愈合能力,并抑制细胞模型中NF-κB/NLRP3通路介导的细胞焦亡相关蛋白和mRNA的表达。结论 在脂多糖诱导的SV-HUC-1细胞间质性膀胱炎体外模型中,羟基积雪草苷可通过抑制NF-κB/NLRP3通路介导的细胞焦亡从而发挥对膀胱上皮细胞的保护作用。Objective To determine the potential effect of madecassoside on interstitial cystitis(IC)through the pyroptosis mediated by NF-κB/NLRP3.Methods An in vitro IC model induced by lipopolysaccharide in SV-HUC-1 cells was used to evaluate the effect of different concentrations of madecassoside.The protective effects of madecassoside on human ureteral epithelial cells were assessed with the MTT cell viability assay and single-layer cell scratch assay.The inhibitory effects of madecassoside on NF-κB/NLRP3-mediated pyroptosis were investigated through Western blot and real-time PCR analysis.Results Madecassoside enhanced the cell viability and wound healing compared to the model group in vitro.It effectively suppressed the expression of pyroptosis-related proteins and mRNA mediated by the NF-κB/NLRP3 pathways in the cell model.Conclusion Madecassoside demonstrates a protective role in the lipopolysaccharide induced SV-HUC-1 cell model of IC by inhibiting pyroptosis through the NF-κB/NLRP3 pathways.

关 键 词：间质性膀胱炎 羟基积雪草苷 NF-κB/NLRP3 细胞焦亡 

分 类 号：R285[医药卫生—中药学]