黄瓜嫩果果皮颜色QTL定位与候选基因分析  

QTL Mapping and Candidate Gene Analysis of Cucumber Immature Fruit Skin Color

作　　者：李晨曦 董邵云[2] 李锦斌 刘小萍 官健涛 苗晗[2] 徐奎鹏 张圣平[2] LI Chenxi;DONG Shaoyun;LI Jinbin;LIU Xiaoping;GUAN Jiantao;MIAO Han;XU Kuipeng;ZHANG Shengping(College of Horticulture,Qingdao Agricultural University,Qingdao 266000,Shandong,China;State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]青岛农业大学园艺学院,山东青岛266000 [2]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081

出　　处：《中国蔬菜》2024年第6期39-47,共9页China Vegetables

基　　金：国家现代农业产业技术体系项目(CARS-23);中国农业科学院创新工程项目(CAAS-ASTIP-2017-IVF);农业农村部园艺作物生物学与种质创制重点实验室项目。

摘　　要：果皮颜色是黄瓜(Cucumis sativus L.)重要的外观品质性状。以黄瓜嫩果绿色果皮材料CSIVF0254和嫩果白色果皮材料CSIVF0021为亲本,构建F2群体,对黄瓜嫩果果皮颜色基因进行遗传规律分析和基因定位研究。结果表明黄瓜嫩果果皮绿色对白色为不完全显性。2022年春季和秋季均检测到相同的主效位点Qcsc3.1、Qcsc5.1,其中Qcsc3.1被定位到3号染色体标记InDel3842~InDel4076之间,LOD值为17.19和24.92,表型贡献率在36.38%~39.86%之间;Qcsc5.1被定位到5号染色体标记InDel2342~SSR06660之间,LOD值为13.51和23.11,表型贡献率在28.53%~34.19%之间。通过生物信息学和qRT-PCR分析预测到5个候选基因。研究结果为调控果皮颜色基因精细定位及分子标记辅助选择育种奠定了良好的基础。The skin color of immature fruit is an important external trait of cucumber(Cucumis sativus L.).An F2 population was constructed with the cucumber green immature fruit color material CSIVF0254 and white immature fruit color material CSIVF0021 as the parental lines to study the inheritance pattern and gene mapping of the cucumber immature fruit pericarp color genes.The results showed that green immature fruit color was incompletely dominant to white immature fruit color,and the major loci Qcsc3.1 and Qcsc5.1 were repeatedly detected in the spring and autumn of 2022.Qcsc3.1 was localized between markers InDel3842-InDel4076 on chromosome 3,with the LOD value of 17.19 and 24.92,and contribution rates of 36.38%-39.86%.Qcsc5.1 was mapped between markers InDel2342-SSR06660 on chromosome 5,with the LOD value of 13.51 and 23.11,and contribution rates of 28.53%-34.19%,respectively.Five genes were predicted via biological information analysis and qRT-PCR analysis.The results of this study can lay a good foundation for the fine mapping of genes regulating fruit skin color and molecular marker-assisted selection and breeding.

关 键 词：黄瓜 果皮颜色 F2群体 QTL定位 

分 类 号：S642.2[农业科学—蔬菜学]