50份加工型辣椒DH系苗期耐低温弱光综合评价  

Comprehensive Evaluation of Low Temperature and Weak Light Tolerance of 50 Processing Pepper Doubled Haploid at Seedling Stage

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作  者:王春萍[1] 李怡斐[1] 张世才[1] 杨小苗 段敏杰 雷开荣[1] 黄启中[1] 黄任中[1] WANG Chunping;LI Yifei;ZHANG Shicai;YANG Xiaomiao;DUAN Minjie;LEI Kairong;HUANG Qizhong;HUANG Renzhong(Chongqing Academy of Agricultural Science,Chongqing Key Laboratory of Adversity Agriculture,Key Laboratory for Evaluation and Utilization of Characteristic Crop Germplasm Resources in Southwest Mountainous Areas(Jointly Established by Departments and Provinces),Chongqing 401329,China)

机构地区:[1]重庆市农业科学院,逆境农业研究重庆市市级重点实验室,西南山地特色作物种质资源评价与利用重点实验室(部省共建),重庆401329

出  处:《中国蔬菜》2024年第6期48-54,共7页China Vegetables

基  金:现代农业产业技术体系专项资金项目(CARS-24-A-04);重庆市自然科学基金项目(CSTB2022NSCQ-MSX0591);重庆市现代农业产业技术体系创新团队专项资助项目;重庆市农业科学院市级财政科研项目(cqaas2023sjczhx016)。

摘  要:以前期利用花药培养技术创制的50份加工型辣椒DH系为试验材料,以SPAD值、F_(v)/F_(m)、根系直径、茎叶干质量、根系干质量、整株干质量和根冠比7个指标的耐低温弱光指数为评价指标,采用模糊隶属函数和聚类分析法对其进行耐低温弱光综合评价。结果表明:50份DH系的耐低温弱光模糊隶属函数综合指数范围在0.20~0.76之间,聚类分析可将其分为两大类,根据耐低温弱光指数特点和模糊隶属函数综合指数可确定第Ⅱ类有7份DH系为耐低温弱光材料,即H201605-8、H201604-4-1、H201612-4-1、H201605-7、H201612-4、H201605-34和H201604-18。50份加工型辣椒DH系间耐低温弱光性差异较大,可作为相关机理研究的试验材料;筛选出的7份耐低温弱光DH系为培育适合设施栽培的优良辣椒新品种提供了材料基础,也为定向培育抗非生物逆境辣椒种质新材料提供了参考。50 pepper DH lines developed through anther culture were used as experimental materials at seedling stage.Comprehensive evaluation of low temperature and weak light resistance was carried out by seven indexes(SPAD value,F_(v)/F_(m),root diameter,dry mass of stem and leaf,dry mass of root,dry mass of whole plant and root-shoot ratio)with fuzzy membership function and cluster analysis.The results showed that the comprehensive index range of the fuzzy membership function for low temperature and weak light tolerance of 50 DH lines is between 0.20 and 0.76,and the 50 DH lines were divided into two categories by cluster analysis.According to the characteristics of low temperature and weak light tolerance,and the comprehensive index of fuzzy membership function,it could be determined that 7 materials in classⅡwere low temperature and weak light resistant materials(H201605-8,H201604-4-1,H201612-4-1,H201605-7,H201612-4,H201605-34 and H201604-18).These results suggested that there is a significant difference in low temperature and weak light tolerance among 50 processing pepper DH lines,which can be used as experimental materials for related mechanisms study.The selected 7 low temperature and weak light tolerance DH lines provide a material basis for cultivating excellent pepper varieties suitable for facility cultivation,and also provide a reference for future targeted cultivation of new biological stress resistant germplasm materials.

关 键 词:辣椒 DH系 低温弱光 模糊隶属函数分析 聚类分析 

分 类 号:S641.3[农业科学—蔬菜学]

 

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