冬瓜苗期立枯病病原鉴定及防治药剂筛选  

Identification of Pathogen Causing Damping off at Seeding Stage of Wax Gourd and Screening of Control Fungicides

作　　者：程亮 秦健 黎炎 莫阳朋 李晶莹 吴永官 CHENG Liang;QIN Jian;LI Yan;MO Yangpeng;LI Jingying;WU Yongguan(Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China;Guangxi Key Laboratory of Vegetable Breeding and New Technology Development,Nanning 530007,Guangxi,China)

机构地区：[1]广西壮族自治区农业科学院蔬菜研究所,广西南宁530007 [2]广西蔬菜育种与新技术研究重点实验室,广西南宁530007

出　　处：《中国蔬菜》2024年第6期74-80,共7页China Vegetables

基　　金：国家自然科学基金项目(32160707);广西科技重大专项(桂科AA22068086-1);广西重点研发计划项目(桂科AB21220029);国家现代农业产业技术体系广西大宗蔬菜创新团队项目(nycytxgxcxtd-2023-10-02);广西农业科学院基本科研业务专项(桂农科2021YT102,桂农科2023YM100)。

摘　　要：为明确广西南宁市冬瓜育苗基地立枯病的病原菌,筛选出高效的杀菌剂,利用组织分离法分离纯化得到立枯病的病原菌,并对14种杀菌剂进行筛选评价。从冬瓜立枯病病样上分离出病原菌,菌株命名为DGLK1,经致病性测定、形态学观察和分子鉴定,确定立枯丝核菌(Rhizoctonia solani AG4HG-Ⅰ)是引起冬瓜立枯病的病原菌,为国内首次报道。参试14种杀菌剂中,450 g·L^(-1)咪鲜胺水乳剂对该病原菌抑制作用最强,EC_(50)为0.2203 mg·L^(-1),其次为500 g·L^(-1)异菌脲悬浮剂、40%五硝·多菌灵可湿性粉剂、430 g·L^(-1)戊唑醇悬浮剂、75%肟菌·戊唑醇水分散粒剂,可作为冬瓜立枯病田间防治的候选药剂。In order to definite the pathogen of damping off at seeding stage of wax gourd from the Nanning region of Guangxi,and screen effective control fungicides,this study isolated and purified the pathogen from damping off at seeding stage of wax gourd by tissue separation method,screend 14 kinds of fungicides,and evaluated their control effects.A fungus was isolated from damping off at seeding stage of wax gourd,and the strain was named as DGLK1.After pathogenicity tests,morphological observation and molecular identification,it was confirmed that Rhizoctonia solani AG4HG-Ⅰwas the causal of damping off on wax gourd seeding,which is reported for the first time in China.Among 14 fungicides,450 g·L^(-1) prochloraz EW had the strongest inhibitory effect with EC_(50) of 0.2203 mg·L^(-1),followed by 500 g·L^(-1) iprodione SC,40%quintozene·carbcndazim WP,430 g·L^(-1) tebuconazole SC,75%trifloxystrobin·tebuconazole WDG.They could be used as candidate agents for field control on damping off on wax gourd seeding.

关 键 词：冬瓜 立枯病 立枯丝核菌 杀菌剂 

分 类 号：S436.429[农业科学—农业昆虫与害虫防治]