GmAP1d regulates flowering time under long-day photoperiods in soybean  

作　　者：Shiyu Guo Yanfei Li Hongmei Qiu Guoyu Hu Chaosen Zhao Ruizhen Wang Hao Zhang Yu Tian Xiaoyu Li Bin Liu Ying-hui Li Li-juan Qiu 

机构地区：[1]College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China [2]State Key Laboratory of Crop Gene Resources and Breeding/The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization(MARA),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]Jilin Academy of Agricultural Sciences/National Engineering Research Center for Soybean,Changchun 130033,Jilin,China [4]Crop Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,Anhui,China [5]Crops Research Institute of Jiangxi Academy of Agricultural Sciences/Jiangxi Province Key Laboratory of Oilcrops Biology,Nanchang 330200,Jiangxi,China [6]Key Lab of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People’s Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China

出　　处：《The Crop Journal》2024年第3期845-855,共11页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(U22A20473);the National Key Research and Development Program of China(2021YFD1201600);the China Agriculture Research System(CARS-04-PS01);the Agricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences,Scientific Innovation 2030 Project(2022ZD0401703);the Platform of National Crop Germplasm Resources of China。

摘　　要：Flowering time is important for adaptation of soybean(Glycine max)to different environments.Here,we conducted a genome-wide association study of flowering time using a panel of 1490 cultivated soybean accessions.We identified three strong signals at the qFT02-2 locus(Chr02:12037319–12238569),which were associated with flowering time in three environments:Gongzhuling,Mengcheng,and Nanchang.By analyzing linkage disequilibrium,gene expression patterns,gene annotation,and the diversity of variants,we identified an AP1 homolog as the candidate gene for the qFT02-2 locus,which we named GmAP1d.Only one nonsynonymous polymorphism existed among 1490 soybean accessions at position Chr02:12087053.Accessions carrying the Chr02:12087053-T allele flowered significantly earlier than those carrying the Chr02:12087053-A allele.Thus,we developed a cleaved amplified polymorphic sequence(CAPS)marker for the SNP at Chr02:12087053,which is suitable for marker-assisted breeding of flowering time.Knockout of GmAP1d in the‘Williams 82’background by gene editing promoted flowering under long-day conditions,confirming that GmAP1d is the causal gene for qFT02-2.An analysis of the region surrounding GmAP1d revealed that GmAP1d was artificially selected during the genetic improvement of soybean.Through stepwise selection,the proportion of modern cultivars carrying the Chr02:12087053-T allele has increased,and this allele has become nearly fixed(95%)in northern China.These findings provide a theoretical basis for better understanding the molecular regulatory mechanism of flowering time in soybean and a target gene that can be used for breeding modern soybean cultivars adapted to different latitudes.

关 键 词：SOYBEAN Flowering time GWAS GmAP1d Long-day conditions 

分 类 号：S565.1[农业科学—作物学]