Identification of new cotton fiber-quality QTL by multiple genomic analyses and development of markers for genomic breeding  

作　　者：Haozhe Tan Binghui Tang Mengling Sun Qiulu Yin Yizan Ma Jianying Li Pengcheng Wang Zhonghua Li Guannan Zhao Maojun Wang Xianlong Zhang Chunyuan You Lili Tu 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,Hubei,China [2]Cotton Research Institute,Shihezi Academy of Agriculture Science,Shihezi 832000,Xinjiang,China

出　　处：《The Crop Journal》2024年第3期866-879,共14页作物学报（英文版）

基　　金：supported by National Key Research and Development Program of China(2022YFF1001400);the National Natural Science Foundation of China(31830062 and 32172071);Innovation and Application of Superior Crop Germplasm Resources of Shihezi(2021NY01);Breeding of New Cotton Varieties and Application of Transgenic Breeding Technology(2022NY01)。

摘　　要：Cotton fiber is one of the main raw materials for the textile industry.In recent years,many cotton fiber quality QTL have been identified,but few were applied in breeding.In this study,a genome wide association study(GWAS)of fiber-quality traits in 265 upland cotton breeding intermediate lines(GhBreeding),combined with genome-wide selective sweep analysis(GSSA)and genomic selection(GS),revealed 25 QTL.Most of these QTL were ignored by only using GWAS.The CRISPR/Cas9 mutants of GhMYB_D13 had shorter fiber,which indicates the credibility of QTL to a certain extent.Then these QTL were verified in other cotton natural populations,5 stable QTL were found having broad potential for application in breeding.Additionally,among these 5 stable QTL,superior genotypes of 4 showed an enrichment in most improved new varieties widely cultivated currently.These findings provide insights for how to identify more QTL through combined multiple genomic analysis to apply in breeding.

关 键 词：Cotton fiber quality breeding GWAS Genome-wide selective sweep analysis Genomic selection InDel markers 

分 类 号：S562[农业科学—作物学]