A rice exocyst subunit gene SEC3A is required for anther dehiscence  

作　　者：Ranran Tu Hong Wang Zhihao Sun Qinwen Zou Jiajun Wu Duo Wu Nan Wang Guanghua He 

机构地区：[1]Rice Research Institute,Key Laboratory of Application and Safety Control of Genetically Modified Crops,Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China

出　　处：《The Crop Journal》2024年第3期938-943,共6页作物学报（英文版）

基　　金：supported by the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(32171970);the Chongqing Outstanding Scientists Project(cstc2022ycjh-bgzxm0073);the Natural Science Foundation of Chongqing,China(cstc2021jcyj-cxttX0004)。

摘　　要：Anther dehiscence controls optimal interaction between pollen and stigma,thereby determining the successful sexual reproduction.The regulators or mechanisms of this process remain elusive.Here,two CRISPR/Cas9 mutants of a rice exocyst subunit gene SEC3A,sec3a-1 and sec3a-2,showed anther indehiscence at anthesis and male sterility at maturity.Pollen viability and germination in the mutants were partly defective,whereas their female gametes undergone a normal development.Hybrid or self-pollinated seeds could be produced by artificial pollination,suggesting potential use of a weak sec3a mutant as a female line during hybrid breeding.SEC3A is widely expressed in various tissues,including anther walls.Further results showed an excessive IAA accumulation and no endothecium lignification in sec3a-1/2 anthers.Our findings suggest that SEC3A appears to regulate anther dehiscence by modulating auxin signaling,providing insights into regulation of anther dehiscence and function of exocyst in plants.

关 键 词：RICE Male sterility Anther dehiscence EXOCYST SEC3A 

分 类 号：S511[农业科学—作物学]