骨碎补大棚栽培品与野生品的广靶代谢组学比较分析  

Comparative Analysis of Broad-targeted Metabolomics Between Greenhouse Cultivated and Wild Collected Drynaria fortune

作　　者：邱道寿[1] 罗永坚 李长发 杜长江 袁雪平 QIU Daoshou;LUO Yongjian;LI Changfa;DU Changjiang;YUAN Xueping(Crops Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Crop Genetic Improvement,Guangzhou 510640,China;School of Life Science and Technology,Central South University of Forestry and Technology,Changsha 410004,China;Guangdong Yangfan Technology Agriculture Co.Ltd.,Renhua 512399,China;Wengyuan County Hengzhiyuan Agriculture and Forestry Technology Co.,Ltd.,Wengyuan 512637,China)

机构地区：[1]广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室,广东广州510640 [2]中南林业科技大学生命科学与技术学院,湖南长沙410004 [3]广东杨帆科技农业有限公司,广东仁化512399 [4]翁源县恒之源农林科技有限公司,广东翁源512637

出　　处：《广东农业科学》2024年第5期30-43,共14页Guangdong Agricultural Sciences

基　　金：广东省科技计划项目(2019B030316008,2023B1212060038);广东省科技专项资金项目(200831086270718);广东省林业科技创新项目(2021KJCX013)。

摘　　要：【目的】骨碎补是一种多年生传统中药材,目前药材原料多来源于野生采集,鲜见有人工栽培的报道。因过度采集,骨碎补野生资源破坏严重,导致药材资源锐减,人工种植成为必然趋势。在探索骨碎补设施大棚栽培技术的基础上,利用代谢组学技术开展大棚栽培药材与野生药材的靶向代谢组学比较研究,探讨栽培品与野生品主要代谢产物的差异,为骨碎补人工栽培技术推广提供依据。【方法】以骨碎补基源植物槲蕨为研究对象,采用UPLC-MS/MS分析技术对比分析设施大棚栽培3年的槲蕨根茎与其野生根茎之间的差异代谢物,并开展差异代谢物富集通路分析。【结果】从两种药材检测到的749种代谢物中筛选到100种差异代谢物,且与野生品相比,大棚栽培品中有58种代谢物含量上调、42种含量下调。这些差异代谢物主要包括黄酮类化合物、有机酸、氨基酸及其衍生物、酚酸类、生物碱、游离脂肪酸等。在大棚栽培品中,黄酮醇、黄酮碳糖苷、糖及糖类代谢物含量全部上调;而在野生品中,氨基酸及衍生物、酚酸类、溶血磷脂酰胆碱和花青素类代谢物含量上调幅度较大。通路富集分析结果显示,100个差异代谢物共注释到47条代谢通路,其中显著富集的有维生素B6代谢、芪类化合物生物合成、二芳基庚烷生物合成、姜酚的生物合成、黄酮和黄酮醇生物合成、半胱氨酸代谢、蛋氨酸代谢、花青素生物合成、氨酰基tRNA生物合成等途径。【结论】大棚栽培的骨碎补,其黄酮醇、黄酮碳糖苷、糖及糖类代谢物化合物含量显著高于野生品,因总黄酮被公认为主要药效成分,因此大棚栽培的骨碎补比野生品具有主效成分优势,可为骨碎补规模化设施栽培或人工栽培提供科学依据。【Objective】Drynaria fortunei is a perennial traditional Chinese medicinal herb.Currently,most medicinal materials of this herb are obtained through wild collection,with few reports on artificial cultivation.Due to excessive collection,the wild resources of D.fortune have been severely damaged,leading to a significant decline in medicinal resources.As a result,artificial cultivation has become an inevitable trend.On the basis of exploring the greenhouse cultivation technology of D.fortune,a targeted metabolomics comparative study was conducted between greenhouse-cultivated rhizomes and wild rhizomes by using metabolomics technology.The study aimed to explore the differences in the main metabolites between cultivated and wild rhizomes to provide a basis for the promotion of artificial cultivation technology of D.fortune.【Method】Taking the original plant of Rhizoma Drynariae,D.fortune as research object,and UPLC-MS/MS analysis technology was utilized to compare and analyze the differential metabolites between three-year greenhouse-cultivated rhizomes and three-year wild rhizomes.Subsequently,enrichment pathway analysis of differential metabolites was conducted by the KEGG pathway database.【Result】Among 749 metabolites detected from two D.fortune samples,100 differential metabolites were screened.Compared with wild samples,there were 58 upregulated metabolites and 42 downregulated metabolites in greenhouse-cultivated samples.These differential metabolites mainly included flavonoids,organic acids,amino acids and their derivatives,phenolic acids,alkaloids,free fatty acids and other metabolites.In greenhouse-cultivated samples,flavonols,flavonoid glycosides,sugars and carbohydrate metabolites were all upregulated.Conversely,in wild samples,amino acids and their derivatives,phenolic acids,lysophosphatidylcholine and anthocyanin metabolites were upregulated to a greater extent.Further pathway enrichment analysis revealed that 100 differential metabolites were annotated into 47 metabolic pathways.Significantly

关 键 词：骨碎补 槲蕨 大棚栽培 野生药材 代谢组学 黄酮 

分 类 号：S58[农业科学—作物学] R282.2[医药卫生—中药学]