^(32)P不同温度不同照射剂量对SD大鼠放射性皮肤损伤初步研究  

Preliminary study on radiation skin injury of SD rats at different temperature and dose of ^(32)P

作　　者：郭佳 程凤 曹滨验 游平飞 王秋 GUO Jia;CHENG Feng;CAO Bin-yan;YOU Ping-fei;WANG Qiu(Department of Nuclear Medicine,General Hospital of Northern Theater Command,Shenyang 110016,China;Department of Emergency Medicine,General Hospital of Northern Theater Command,Shenyang 110016,China;Dalian Medical University,Dalian 116000,China;China Medical University,Shenyang 110000,China)

机构地区：[1]北部战区总医院核医学科,辽宁沈阳110016 [2]北部战区总医院急诊医学科,辽宁沈阳110016 [3]大连医科大学,辽宁大连116000 [4]中国医科大学,辽宁沈阳110000

出　　处：《临床军医杂志》2024年第5期479-482,共4页Clinical Journal of Medical Officers

基　　金：军队后勤科研重点课题(BLB19J012);军队护理创新与培育专项计划(2021HL057)。

摘　　要：目的探讨^(32)P不同温度不同照射剂量造成SD大鼠放射性皮肤损伤的病理状态。方法选取48只6~8周雄性SD清洁级大鼠为研究对象。采用随机数字表法将48只大鼠随机分为A组(室温下10 Gy)、B组(低温下10 Gy)、C组(室温下30 Gy)、D组(低温下30 Gy)、E组(室温下60 Gy)、F组(低温下60 Gy),每组各8只。采用^(32)P发射的β射线进行照射。计算大鼠皮肤损伤面积、进行皮肤损伤评分及病理切片苏木素伊红染色。比较各组大鼠不同照射时间皮肤损伤面积百分比、皮肤损伤评分及病理学变化。结果F组大鼠不同照射时间的皮肤损伤面积百分比均高于E组,差异均有统计学意义(P<0.05)。D组照射后1周、照射后2周的皮肤损伤评分均高于C组,差异均有统计学意义(P<0.05)。低温中的B组、D组、F组大鼠受照皮肤的病理改变均早于室温下的A组、C组、E组,且损伤程度较重。结论低温环境下的皮肤β射线损伤发生早、损伤进展快,且其损伤程度随照射剂量增加而加重。Objective To investigate the pathological status of radiation skin injury in SD rats caused by different temperature and dose of^(32)P.Methods Forty-eight male SD clean rats were selected as the study subjects.A total of 48 rats were randomly divided into group A(10 Gy at room temperature),group B(10 Gy at low temperature),group C(30 Gy at room temperature),group D(30 Gy at low temperature),group E(60 Gy at room temperature)and group F(60 Gy at low temperature)with 8 rats in each group.It was irradiated by^(32)P beta rays.The skin injury area of rats was calculated,the skin injury score was performed and the pathological sections were stained with hematoxylin eosin.The percentage of skin damage area,skin damage score and pathological changes of rats in different irradiation time were compared.Results The percentage of skin damage area in group F was higher than that in group E at different irradiation times,and the differences were statistically significant(P<0.05).The skin injury scores of group D 1 week and 2 weeks after irradiation were higher than those of group C,and the differences were statistically significant(P<0.05).The pathological changes of exposed skin of rats in groups B,D and F at low temperature were earlier than those in groups A,C and E at room temperature,and the damage degree was more serious.Conclusion The skinβ-ray damage occurs early and progresses rapidly in low temperature environment,and the damage degree increases with the increase of irradiation dose.

关 键 词：照射剂量 放射性皮肤损伤 Β射线 皮肤损伤评分 

分 类 号：R818[医药卫生—放射医学] R332[医药卫生—临床医学]