油橄榄压条不定根发生的转录组分析  

Transcriptome Analysis on Formation of Adventitious Root during Olive Horizontal Press Seedling

作　　者：赵敏 刘坤 王毅 胡青 邸多隆[2] 裴栋[2] 陆斌 ZHAO Min;LIU Kun;WANG Yi;HU Qing;DI Duolong;PEI Dong;LU Bin(Yunnan Academy of Forestry and Grassland,Kunming Yunnan 650201,P.R.China;CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou Gansu 730000,P.R.China)

机构地区：[1]云南省林业和草原科学院,云南昆明6502012 [2]中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州730000

出　　处：《西部林业科学》2024年第3期68-77,共10页Journal of West China Forestry Science

基　　金：云南省种子种业联合实验室项目“适生油橄榄品种选育”(202205AR070001-16);云南省科技厅高层次科技人才及创新团队选拔专项“技术创新人才培养对象项目”(202205AD160016)。

摘　　要：探明油橄榄生根过程中基因表达调控的变化规律,可为控制不定根发生的关键基因的筛选奠定基础。以油橄榄品种‘田园1号’为研究对象,采用生根粉1号(1500 mg/kg)处理油橄榄压条,并在压条后的第0、10、20、30和40 d取样(记为YT0、YT10、YT20、YT30、YT40)进行转录组测序,分析油橄榄生根过程不同阶段的差异基因的表达变化,挖掘出参与油橄榄生根过程的重要代谢通路及生根相关差异表达基因。结果显示:(1)压条生根过程中差异表达基因在YT0与YT10间共1218个,包括829个上调和389个下调;在YT10与YT20间共有1157个,包括567个上调和590个下调;在YT20与YT30间共1163个,包括499个上调和664个下调;在YT30与YT40间共1458个,包括952个上调和506个下调。(2)通过差异基因GO功能注释分析发现显著富集的类名包括细胞膜、催化活性、氧化还原酶活性、辅因子结合、转运活性、跨膜转运蛋白活性、碳水化合物代谢过程等。(3)通过差异基因KEGG富集分析,发现参与生根过程的主要通路包括植物激素信号转导、苯丙烷生物合成、淀粉和蔗糖代谢、戊糖和葡萄糖醛酸相互转化、氨基糖和核苷酸糖、核糖体、半胱氨酸和蛋氨酸代谢等。结果表明:在压条生根过程中,与植物激素信号转导有关的基因包括ARF、GH 3、AUX1、SAUR、JAZ、CYCD3、MYC 2等;与苯丙烷生物合成途径相关的基因包括β-葡萄糖苷酶、反-肉桂酸4-单加氧酶、苯丙氨酸解氨酶等;参与生根过程的转录因子包括bHLH、MYB、ERF、NAC、C2H2和WRKY等家族。Exploring the changes in gene expression regulation during the rooting process of olive(Olea europaea)could lay a foundation for screening key genes that control adventitious root development.Using the olive variety O.europaea‘Tianyuan 1’as the experimental object,rooting powder 1(1500 mg/kg)was used to treat olive cuttings.The rooting process was observed and transcriptome sequencing was performed on samples taken at 0,10,20,30 and 40 days after the cuttings were pressed.The expression of differentially expressed genes at different stages of olive rooting process was analyzed,and important metabolic pathways involved in olive rooting process were identified.The results showed that:(1)There were 1218 genes(P<0.05)with significant differences in expression during the rooting process between YT0 and YT10,including 829 up-regulated and 389 down-regulated genes;Between YT10 and YT20,there are a total of 1157,including 567 up-regulated and 590 down-regulated;Between YT20 and YT30,there are a total of 1163,including 499 up-regulated and 664 down-regulated;There are a total of 1458 between YT30 and YT40,including 952 up-regulated and 506 down-regulated.(2)Through differential gene GO functional annotation analysis,it was found that significantly enriched terms include cell membrane,catalytic activity,oxidoreductase activity,cofactor binding,transport activity,transmembrane transport protein activity,carbohydrate metabolism processes,etc.(3)By analyzing the KEGG enrichment analysis of differentially expressed genes,it was found that the main pathways involved in the rooting process include plant hormone signal transduction,phenylpropanoid biosynthesis,starch and sucrose metabolism,conversion of pentose and glucuronic acid,amino and nucleotide sugars,ribosomes,cysteine and methionine metabolism,amino and nucleotide sugars,etc.The results indicate that genes related to plant hormone signal transduction during the rooting process include ARF,GH3,AUX1,SAUR,JAZ,CYCD3,MYC2,etc.while genes related to phenylpropanoid biosynt

关 键 词：油橄榄 压条 不定根形成 转录组学 

分 类 号：S565.7[农业科学—作物学]