高温胁迫下水稻胚乳磷酸化与泛素化蛋白质组学联合分析  

Integrated analysis of phosphoproteome and ubiquitinated proteome in rice endosperm under high temperature stress

作　　者：庞悦涵 应逸宁 徐非非 包劲松[1,2] PANG Yuehan;YING Yining;XU Feifei;BAO Jinsong(Institute of Nuclear Agricultural Sciences,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,Zhejiang,China;Hainan Institute of Zhejiang University,Sanya 572025,Hainan,China)

机构地区：[1]浙江大学农业与生物技术学院原子核农业科学研究所,浙江杭州310058 [2]浙江大学海南研究院,海南三亚572025

出　　处：《浙江大学学报（农业与生命科学版）》2024年第3期382-392,共11页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金项目(32201817);海南省自然科学基金项目(323MS066)。

摘　　要：水稻胚乳的代谢依赖于多个翻译后修饰信号网络的精确调控,包括磷酸化和泛素化修饰。本研究通过磷酸化与泛素化蛋白质组学的联合分析,发现水稻胚乳中有143个蛋白质同时具有磷酸化和泛素化位点,这为研究磷酸化和泛素化修饰之间的串扰机制提供了组学基础。一方面,磷酸化修饰通过影响泛素-蛋白酶体系统中的关键蛋白来调控泛素化修饰;另一方面,泛素化修饰通过影响蛋白激酶和蛋白磷酸酶来间接调控植物体内的磷酸化信号网络。高温胁迫下,磷酸化和泛素化修饰协同调控酚类化合物的合成通路,并最终影响酚类化合物清除活性氧的能力以及植物在逆境中的抗性。多种淀粉代谢相关酶同时具有磷酸化和泛素化修饰,并且有18对具有不同修饰类型的位点出现在同一蛋白质的相邻位置,揭示了不同修饰之间的串扰机制以及高温胁迫中磷酸化与泛素化修饰对淀粉代谢的协同调节作用。The metabolism of rice endosperm depends on the precise regulation of several post-translational modification(PTM)-based signaling networks,including phosphorylation and ubiquitination modifications.This study,integrating analysis of proteins from phosphoproteome and ubiquitinated proteome,identified 143 proteins with both phosphorylated and ubiquitinated sites,providing a basis for understanding the crosstalk mechanism between phosphorylation and ubiquitination modifications in rice endosperm.On the one hand,phosphorylation modification of key proteins involved in the ubiquitin-proteasome system contributed to the regulation of ubiquitination modification;on the other hand,ubiquitination modification of protein kinases and phosphatases might affect the phosphorylated signaling network.Under high temperature stress,phosphorylation and ubiquitination modifications jointly regulated the synthesis pathway of phenolic compounds,ultimately affecting the ability of phenolic compounds to scavenge reactive oxygen species and plant resistance to stress.Several enzymes related to starch metabolism possessed both phosphorylation and ubiquitination modifications,and 18 pairs of sites with different modification types appeared adjacent to the same protein,revealing the crosstalk mechanism between different modifications and the synergistic regulation of starch metabolism by phosphorylation and ubiquitination modifications under high temperature stress.

关 键 词：水稻胚乳 高温胁迫 磷酸化 泛素化 淀粉生物合成 

分 类 号：S511.2[农业科学—作物学]