基于磁压榨技术原理的直肠阴道瘘动物模型制备方法的优化  

Optimization of preparation method of rectovaginal fistula animal model based on principle of magnetic compression technique

作　　者：田波彦 张苗苗[1,2] 冒健骐 吕毅 严小鹏[1,2] TIAN Boyan;ZHANG Miaomiao;MAO Jianqi;LYU Yi;YAN Xiaopeng(Department of Hepatobiliary Surgery,First Affiliated Hospital of Xi’an Jiaotong University,Xi’an,Shaanxi 710061,China;National Local Joint Engineering Research Center for Precision Surgery&Regenerative Medicine,First Affiliated Hospital of Xi’an Jiaotong University,Xi’an,Shaanxi 710061,China;Zonglian College,Xi’an Jiaotong University,Xi’an,Shaanxi 710061,China)

机构地区：[1]西安交通大学第一附属医院肝胆外科,西安710061 [2]西安交通大学第一附属医院精准外科与再生医学国家地方联合工程研究中心,西安710061 [3]西安交通大学宗濂书院,西安710061

出　　处：《重庆医学》2024年第12期1768-1771,1777,共5页Chongqing medicine

基　　金：中央高校基本科研业务费专项基金项目(xzy022023068);西安交通大学第一附属医院科研发展基金项目(2022MS-07)。

摘　　要：目的探讨基于磁压榨技术原理优化设计的“工”形结构磁体用于直肠阴道瘘动物模型制备的可行性。方法以10只新西兰雌兔作为模型动物,麻醉后将两枚自行设计的磁体分别经阴道和肛门置入,调整两枚磁体于合适位置并使其相吸,形成磁体-直肠阴道隔-磁体的结构。当磁体间受压组织缺血坏死并脱落后,两枚磁体形成“工”形结构并位于直肠阴道瘘瘘口内,起到阻止瘘口变小和自行愈合的目的。记录手术操作时间、直肠阴道瘘形成时间;术后2周处死实验兔获取直肠阴道瘘标本,观察瘘口形成情况,测量瘘口大小。结果10只实验兔均成功制备了直肠阴道瘘动物模型,术中磁体置入过程顺利,手术操作时间(1.55±0.65)min,术后实验动物一般状况良好,术后(4.80±0.75)d直肠阴道瘘瘘口形成。术后2周获取直肠阴道隔大体标本后取出磁体,可见直肠阴道瘘瘘口形成良好,瘘口直径(5.86±0.38)mm。结论基于磁压榨技术原理设计的“工”形结构磁体可用于直肠阴道瘘动物模型制备,其操作简单、模型制备成功率高、瘘口大小均一性好。Objective To investigate the feasibility of the optimally designed“I”shaped structure magnet based on the principle of magnetic compression technique for the preparation of rectovaginal fistula animal model.Methods Using 10 New Zealand female rabbits as the model animals,two self-designed magnets were inserted through the vagina and anus respectively after anesthesia,and the two magnets were adjusted to the appropriate position and made them attraction each other to form a magnet-rectovaginal partition-magnet structure.When the compression tissue between the magnets became ischemic necrosis and fell off,the two magnets formed the“I”shape structure and were located in the stoma of rectovaginal fistula to prevent the stoma from becoming smaller or even closing itself.The operation time and rectovaginal fistula formation time were recorded.The experimental rabbits were killed in postoperative 2 weeks,and the rectovaginal fistula specimens were obtained.The formation of fistula orifice was observed and the size of fistula orifice was measured.Results The animal model of rectovaginal fistula was successfully prepared in all 10 experimental rabbits.The procedure of intraoperative magnet placement was smooth and the operation time was(1.55±0.65)min.The experimental animals were generally in good condition after surgery,and the fistula orifice was formed on postoperative(4.80±0.75)d.After taking the gross specimen of rectovaginal septum in postoperative 2 weeks,the magnet was removed.The fistula orifice of rectovaginal fistula was visible with the diameter of(5.86±0.38)mm.Conclusion The“I”shaped structure magnet designed based on the principle of magnetic compression technique could be used in the preparation of the rectovaginal fistula animal model.Its operation is simple with high success rate of model preparation and good uniformity in fistula orifice.

关 键 词：磁压榨技术 磁外科 直肠阴道瘘 磁体 动物模型 

分 类 号：R656[医药卫生—外科学]