Limit Test and pH Determination of 5-Hydroxymethylfurfural in Jiulongteng Honey  

作　　者：Beiqiao YIN Yanyan CHEN Lili LIU Shenggao YIN Yanxia CEN Shiman CHEN 

机构地区：[1]Baise People's Hospital,Baise 533000,China [2]Wuzhou Vocational College,Wuzhou 543003,China [3]Guangxi University of Chinese Medicine,Nanning 530000,China [4]Beihai Public Inspection and Testing Center,Beihai 536000,China

出　　处：《Medicinal Plant》2024年第3期23-25,29,共4页药用植物：英文版

基　　金：Supported by Young and Middle-aged Teachers Scientific Research Basic Ability Improvement Project in Universities of Guangxi in 2020 (2020 KY07040);School-level Scientific Research Project of Guangxi University of Chinese Medicine in 2021 (2021MS010).

摘　　要：[Objectives]To explore the effect of storage time on pH and 5-hydroxymethylfurfural content in Jiulongteng honey.[Methods]The pH of Jiulongteng honey was determined by neutralization titration with sodium hydroxide standard solution.The content of 5-hydroxymethylfurfural in Jiulongteng honey was determined by HPLC.Chromatographic conditions:ZORBAX SB-C 18 column(250 mm×4.6 mm,5μm)from Agilent Co.,Ltd.,acetonitrile-0.1%formic acid solution(5:95)as mobile phase,flow rate of 0.8 mL/min,5-hydroxymethylfurfural detection wavelength of 284 nm,guanosine detection wavelength of 254 nm.[Results]The pH of 12 batches of Jiulongteng honey was 3.70-3.84 in the new honey stage,3.92-4.05 in the old honey stage 1,and 4.25-4.53 in the old honey stage 2;5-hydroxymethylfurfural was not detected in the new honey stage,5-hydroxymethylfurfural was detected in FM-001 in the old honey stage 1,and 5-hydroxymethylfurfural was detected in most samples in the old honey stage 2.[Conclusions]The pH and 5-hydroxymethylfurfural content of 12 batches of Jiulongteng honey met the requirements within 3 years of storage.There was no 5-hydroxymethylfurfural in Jiulongteng honey,but with the extension of storage time,the detection amount of 5-hydroxymethylfurfural increased significantly even if Jiulongteng honey was stored at low temperature.Therefore,5-hydroxymethylfurfural can be used as an important indicator of honey freshness.

关 键 词：Jiulongteng HONEY 5-HYDROXYMETHYLFURFURAL PH HPLC 

分 类 号：R28[医药卫生—中药学]