TLC Identification and Extraction Process of Rubiasin-1-methyl Ether from Yao Medicine Chuanlianzhu  

作　　者：Jingrong LU Jiangcun WEI Xiumei MA Bing QING Meiyan QIU Wen ZHONG 

机构地区：[1]Guangxi International Zhuang Medicine Hospital,Nanning 530201,China

出　　处：《Medicinal Plant》2024年第3期35-38,共4页药用植物：英文版

基　　金：Supported by State Administration of Traditional Chinese Medicine High-level Key Discipline Construction Project of Traditional Chinese Medicine-Ethnic Minority Pharmacy (Zhuang Pharmacy) (zyyzdxk-2023165);General Scientific Research Program of Guangxi University of Chinese Medicine in 2020 (2020MS063);Key R&D Project of Guangxi Science and Technology Department (Guike AB21196057);Young Talent Cultivation Program of Guangxi International Zhuang Medicine Hospital (2022001);Funding Project of High-level Talent Cultivation and Innovation Team of Guangxi University of Chinese Medicine (2022A008);Guangxi Traditional Chinese Medicine Interdisciplinary Innovation Team Project (GZKJ2309);State Administration of Traditional Chinese Medicine"Twelfth Five-Year Plan"Key Discipline of Traditional Chinese Medicine (Ethnic Pharmacy)Zhuang Pharmacy.

摘　　要：[Objectives]To establish a thin-layer chromatography(TLC)method for the determination of rubiadin-1-methyl ether in Yao Medicine Chuanlianzhu(Damnacanthus giganteus).[Methods]A silica gel G thin-layer plate was adopted for TLC.Petroleum ether(60-90℃)-chloroform-methanol-water(7:15:3:1)was used as the developing solvent and inspected under ultraviolet lamp(365 nm).The content was determined by Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm),mobile phase:acetonitrile-0.2%phosphoric acid gradient elution,detection wavelength 277 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The spots of 10 Chuanlianzhu samples from different origins showed the same color at the same position as the control,and the spots were clear and specific.The injection volume of rubiadin-1-methyl ether showed a good linear relationship in the range of 2.90-145μg(R=0.9996).The average recovery rate of rubiadin-1-methyl ether in the low,medium and high dose groups of Yao Medicine Chuanlianzhu was 98.72%,and RSD=1.78%.[Conclusions]This method can effectively identify Yao Medicine Chuanlianzhu medicinal materials and accurately determine the content of rubiadin-1-methyl ether in the medicinal materials.It provides a scientific basis for the development and utilization of Yao Medicine Chuanlianzhu medicinal resources.

关 键 词：Chuanlianzhu THIN-LAYER chromatography (TLC) Extraction process Rubiadin-1-methyl ETHER Content determination 

分 类 号：R284.2[医药卫生—中药学]