Using Network Pharmacology to Explore Therapeutic Effect of Polygonum capitatum on Renal Calculus in Rats  

作　　者：Bo TU Xu ZHANG Yan LIN Huang YANG Minghui HE 

机构地区：[1]Engineering Research Center of Chronic Disease Prevention and Treatment of Authentic Medicinal Materials in Guizhou Higher Education Institutions,School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550025,China [2]State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550004,China

出　　处：《Medicinal Plant》2024年第3期45-49,共5页药用植物：英文版

基　　金：Supported by Guizhou Provincial Science and Technology Plan Project (Gui Qian He Ji Chu-ZK[2022]General 362);Guizhou Provincial Department of Education Science and Technology Achievement Transfer and Transformation Project (Gui Jiao Ji[2022]064);Guizhou Provincial Department of Education Higher Education Engineering Research Center (Gui Jiao Ji[2023]035);China National University Student Innovation&Entrepreneurship Development Program (202210660131&202310660082);Guizhou's Emerging University Think Tank Alliance Construction Project (Qian Jiao Zhe[2023]ZK01).

摘　　要：[Objectives]To explore the therapeutic effect of Polygonum capitatum on renal calculus in rats based on network pharmacology.[Methods]Through the preliminary construction of P.capitatum-urolithiasis disease target network,explore the active components,action pathway and action target of P.capitatum-urolithiasis treatment,and use 1%ethylene glycol+2%ammonium chloride to induce SD rat kidney calcium oxalate stone model to verify the efficacy of P.capitatum-urolithiasis treatment.[Results]Through the network pharmacological prediction,it is found that the important active components in P.capitatum were quercetin,gallic acid,rutin,silybin,catechin,kaempferol and so on;potential active targets include INS,CAT,IL-6,MOCOS,etc.The results also suggest that forkhead transcription factor signaling pathway(FoxO signaling pathway),tumor necrosis factor signaling pathway(TNF signaling pathway)and hypoxia-inducible factor signaling pathway(HIF-1 signaling pathway)are the core pathways.The results of biochemical indicators in animal experiment showed that the contents of serum urea nitrogen(BUN),creatinine(Cr)and malondialdehyde(MDA)in renal tissue in the treatment group(200,500 mg/kg)were significantly lower than those in the model group,while the content of superoxide dismutase(SOD)was significantly higher than that in the model group.In addition,the kidney tissue H&E staining sections showed that P.capitatum alcohol extract administration group rats kidney calcium oxalate crystals were significantly reduced compared with the model group,the degree of renal tubular lumen expansion was lighter than the model group,suggesting that P.capitatum alcohol extract has the effect of improving renal calculus in rats.[Conclusions]This study provides a theoretical reference for the deep development of P.capitatum in the treatment of renal calculus.

关 键 词：POLYGONUM capitatum UROLITHIASIS Network PHARMACOLOGY 

分 类 号：R284.2[医药卫生—中药学]