High nitrogen fertilizer input enhanced the microbial network complexity in the paddy soil  

作　　者：Yanan Chen Yan Li Tianyi Qiu Haoran He Ji Liu Chengjiao Duan Yongxing Cui Min Huang Chunyan Wu Linchuan Fang 

机构地区：[1]The Research Center of Soil and Water Conservation and Ecological Environment,Chinese Academy of Sciences and Ministry of Education,Yangling,712100,China [2]State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau,Institute of Soil and Water Conservation CAS and MWR,Yangling,712100,China [3]University of Chinese Academy of Sciences,Beijing,100049,China [4]Institute of Environment Resource and Soil Fertilizer,Zhejiang Academy of Agricultural Sciences,Hangzhou,310021,China [5]College of Natural Resources and Environment,Northwest A&F University,Yangling,712100,China [6]Hubei Province Key Laboratory for Geographical Process Analysis and Simulation,Central China Normal University,Wuhan,430079,China [7]Department of Ecohydrology,Leibniz Institute of Freshwater Ecology and Inland Fisheries,Berlin,12587,Germany [8]Sino-French Institute for Earth System Science,College of Urban and Environmental Sciences,Peking University,Beijing,100871,China [9]College of Resources and Environmental Engineering,Wuhan University of Technology,Wuhan,430070,China [10]CAS Center for Excellence in Quaternary Science and Global Change,Xi’an,710061,China

出　　处：《Soil Ecology Letters》2024年第2期97-109,共13页土壤生态学快报（英文）

基　　金：financially supported by the Joint Funds of the National Natural Science of China(U21A20237).

摘　　要：N fertilizer altered bacterial community compositions by changing soil nutrients.•Bacterial ammonia oxidation became predominated with the increasing N rate.•Excessive N input caused the information of a more complex microbial network.•Intensified microbial competition by excessive N was due to negative link increase.Nitrogen(N)fertilization drives the structure and function of soil microbial communities,which are crucial for regulating soil biogeochemical cycling and maintaining ecosystem stability.Despite the N fertilizer effects on soil microbial composition and diversity have been widely investigated,it is generally overlooked that ecosystem processes are carried out via complex associations among microbiome members.Here,we examined the effects of five N fertilization levels(0,135,180,225,and 360 kg N ha−1)on microbial co-occurrence networks and key functional taxa such as ammonia-oxidizers in paddy soils.The results showed that N addition altered microbial community composition,which were positively related to soil total N and available phosphorus(P)contents.The abundance of ammonia-oxidizing archaea(AOA)significantly decreased after N addition,whereas ammonia-oxidizing bacteria(AOB)increased in N360 treatment.Compared with low-N group(N0 and N135),the high-N group(N225 and N360)shaped more complex microbial webs and thus improved the stability of the microbial community.Partial least squares path modeling further revealed that N fertilizer had a higher effect on microbial network complexity in the high-N group(0.83)than the low-N group(0.49).Although there were more positive links across all microbial networks,the proportion of negative links significantly increased in the high-N network,suggesting that excess N addition aggravated the competition among microbial species.Disentangling these interactions between microbial communities and N fertilization advances our understanding of biogeochemical processes in paddy soils and their effects on nutrient supply to rice production.Our findings highlighte

关 键 词：nitrogen fertilizer paddy soil co-occurrence network microbial interaction 

分 类 号：S147[农业科学—肥料学]