两种方法测定饲料添加剂硝酸硫胺(维生素B_(1))含量的比较  

Comparison of two methods to determine the content of thiamine nitrate(vitamin B_1)as feed additive

作　　者：杜言 王博媛 刘小敏 黄昌郡 DU Yan;WANG Bo-yuan;LIU Xiao-min;HUANG Chang-jun(China Grain Wuhan Scientific Research and Design Institute Co.,Ltd.//National Feed Quality Inspection and Testing Center(Wuhan),Wuhan 430079,China)

机构地区：[1]国粮武汉科学研究设计院有限公司//国家饲料质量检验检测中心(武汉),湖北武汉430079

出　　处：《粮食与饲料工业》2024年第3期66-69,共4页Cereal & Feed Industry

摘　　要：分析比较了硅钨酸沉淀法和电位滴定法在硝酸硫胺(维生素B_(1))含量测定上的差异,以期为实际检测工作提供参考。分别用两种方法在0、3、6、9、12、18、24、36 d后测定硝酸硫胺含量,硅钨酸沉淀法的RSD为0.1%,电位滴定法的RSD为0.1%,两种方法的稳定性均良好。分别采用两种方法测定3个企业不同批次的样品42个,硝酸硫胺含量(干基计)硅钨酸沉淀法与电位滴定法均在98.0%~101.0%;硅钨酸沉淀法与电位滴定法两次独立测定结果的绝对差值均小于0.4%,两种方法测定结果差异不显著(P>0.05)。硅钨酸沉淀法耗时长,操作步骤多,但使用试剂和仪器更易得;电位滴定法操作简便快速,准确度高,精密度好,但考虑到饲料企业实际状况和我国对乙酸酐的管制状况,电位滴定法更适用于有条件的生产商、使用方、检验机构使用。The differences between silicotungstic acid precipitation method and potentiometric titration method in the determination of thiamine nitrate(vitamin B_1)content were analyzed and compared,in order to provide reference for practical detection work.After 0,3,6,9,12,18,24 and 36 days,the RSD of silicotungstic acid precipitation method and potentiometric titration method were 0.1%and 0.1%respectively.The stability of the two methods was good.42 samples from different batches of 3 enterprises were determined by two methods,and the contents of thiamine nitrate(dry base meter)were 98.0%~101.0%by silicotungstic acid precipitation method and potentiometric titration method.The absolute difference between the two independent determination results by silicotungstic acid precipitation method and potentiometric titration method was less than 0.4%,and there was no significant difference between the two methods(P>0.05).Silicotungstic acid precipitation method is time-consuming and has many operation steps,but it is easier to use reagents and instruments;Potentiometric titration is simple,rapid,high accuracy and good precision,but considering the actual situation of feed enterprises and the control status of acetic anhydride in China,it is more suitable for conditional manufacturers,users and testing institutions.

关 键 词：硅钨酸沉淀法 电位滴定法 饲料 维生素B_(1) 

分 类 号：S816.17[农业科学—饲料科学]