高效液相色谱法测定疫苗菌体破碎液中D-核糖的技术研究  

Determination of D-ribose in the Bbroken Liquid of Vaccines by High Performance Liquid Chromatography

作　　者：黄婷 徐晓萍 彭雪琦 HUANG Ting;XU Xiaoping;PENG Xueqi(Productivity Centre of Jiangsu Province,Nanjing 210042,China)

机构地区：[1]江苏省生产力促进中心,江苏南京210042

出　　处：《工业微生物》2024年第3期61-64,共4页Industrial Microbiology

基　　金：江苏省生产力促进中心青年人才基金项目“HPV(人乳头瘤病毒)疫苗原液中糖类杂质成分测定技术研究及方法开发”(编号:D2023003)。

摘　　要：文章以示差折光为检测器,建立高效液相色谱法定量测定疫苗菌体破碎液中D-核糖的方法。该方法的测定条件为:色谱柱为安捷伦ZORBAX NH2氨基色谱柱,流动相为0.005 mol/L的稀硫酸溶液,柱温为50℃,进样量为20μL。D-核糖检测方法保留时间为3.684 min,得到的D-核糖的线性回归方程式为y=194.037 72x+10.834 09,相关性为0.999 99。式中,y为D-核糖的质量浓度;x为对应的峰面积。该方法精密度、稳定性和重现性表现良好,D-核糖的回收率范围为90.2%~102%,灵敏度高,能实现目标峰与杂峰的有效分离,因此可被广泛用于疫苗菌体破碎液等样本中D-核糖浓度的测定。A high performance liquid chromatography method for quantitatively determining the D-ribose with refractive index detector(RID)is established.The measurement conditions for this method are as follows:the Agilent ZORBAX NH2 column,0.005 mol/L of diluted sulfuric acid solution as the mobile phase,50℃ column temperature and the 20μL injection volume.The retention time of D-ribose is 3.684 min.The linear regression equation for obtaining D-ribose is y=194.03772x+10.83409,and the correlation is 0.99999,in which the y means the mass concentration of D-ribose and the x means the corresponding peak area.This method exhibits good precision,stability,and reproducibility.The recovery of D-ribose ranges from 90.2%to 102%.This improved method has high sensitivity and can achieve effective separation of target peak and impurity peak.Therefore,it can be widely used for measuring the D-ribose levels in a variety of the broken liquid of vaccines.

关 键 词：D-核糖 示差检测器 疫苗菌体破碎液 

分 类 号：O657.72[理学—分析化学] R392-33[理学—化学]