提高维生素C二步发酵种子质量的方法  

Methods of improving inoculum quality of vitamin C two-step fermentation

作　　者：胡静[1,3,4] 刘丽华 朱娜[2] 苏宏儒 王婧 米帅东 Hu Jing;Liu Lihua;Zhu Na;Su Hongru;Wang Jing;Mi Shuaidong(CSPC Weisheng Pharmaceutical(Shijiazhuang)Corporation Ltd.,Shijiazhuang 050000,China;Shijiazhuang Food and Drug Inspection Center Shijiazhuang 050000,China;Shijiazhuang Vitamin C Technology Innovation Center,Shijiazhuang 050000,China;Hebei Province Nutrition Fortifier Technology Innovation Center,Shijiazhuang 050000 China)

机构地区：[1]石药集团维生药业(石家庄)有限公司,河北石家庄050000 [2]石家庄市食品药品检验中心,河北石家庄050000 [3]石家庄市维生素C技术创新中心,河北石家庄050000 [4]河北省营养强化剂技术创新中心,河北石家庄050000

出　　处：《煤炭与化工》2024年第5期144-147,共4页Coal and Chemical Industry

摘　　要：通过在维生素C二步种液培养基中添加半胱氨酸或(和)氯化钙的方法,研究了这2种物质对二步菌种生长和产2-酮基-L-古龙酸(2-KLG)的影响。结果表明,在二步种子培养基中加入适量的半胱氨酸和(或)氯化钙能够促进普通生酮基古龙酸菌生长,提高菌体密度,协调混菌比例,提高种子质量,并提高后续发酵2-酮基-L-古龙酸生成速率,缩短发酵周期。在种子培养基中同时加入0.3 g/L半胱氨酸和0.6 g/L氯化钙培养效果最显著:种液中普通生酮基古龙酸菌浓度提高30.77%,发酵周期缩短8.33%,2-酮基-L-古龙酸生成速率提高9.31%。The effects of L-cysteine or(and)calcium chloride on the growth and production of 2-keto-L-gulonic acid(2-KLG)in two-step culture medium of vitamin C were studied.The results showed that adding appropriate amount of L-cysteine and(or)calcium chloride to the two-step culture medium could promote the growth of Ketogulonigenium vulgare,increase the cell density,coordinate the mixed bacteria proportion and improve the quality of the mixed strains,the production rate of 2-keto-L-gulonic acid was increased and the fermentation period was shortened.The effect of adding 0.3 g/L cysteine and 0.6 g/L calcium chloride in the seed liquor culture medium was the most remarkable.The concentration of Ketogulonigenium vulgare in the seed liquor increased by 30.77%,and the fermentation period shortened by 8.33%,the formation rate of 2-keto-L-gulonic acid increased by 9.31%.

关 键 词：二步发酵种子 半胱氨酸 氯化钙 普通生酮基古龙酸菌 菌体密度 2-酮基-L-古龙酸 

分 类 号：TQ466.3[化学工程—制药化工]