海洋放线菌阿维丁链霉菌次生代谢产物研究及杀线虫活性筛选  

Research on Secondary Metabolites and Screening for Nematicidal Activity ofthe Marine Actinomycete Streptomyces avidinii

作　　者：刘杰阳 程琪 唐相琴 范琴玉 王莹 何江波 LIU Jieyang;CHENG Qi;TANG Xiangqin;FAN Qinyu;WANG Ying;HE Jiangbo(School of Medicine,Kunming University,Kunming,Yunnan,China 650214)

机构地区：[1]昆明学院医学院,云南昆明650214

出　　处：《昆明学院学报》2024年第3期82-87,123,共7页Journal of Kunming University

基　　金：云南省教育厅科学研究基金项目(2023J0832,2023Y0870);昆明市春城计划青年拔尖人才项目(C201914001)。

摘　　要：为研究海洋放线菌杀线虫活性物质,通过液体发酵对81株海洋放线菌进行杀线虫活性筛选,然后对活性菌株的发酵液进行分离纯化,并对单体化合物进行杀线虫筛选.筛选出一株海洋放线菌具有较高杀线虫活性,线虫死亡率达到89.9%.该菌株经鉴定为Streptomyces avidinii,并从其发酵液次级代谢产物中得到7个化合物,分别为pyrrole-2-carboxamide(1)、N-(3-(1H-indole-3-yl)propyl)acetamide(2)、p-hydroxybenzyl alcohol(3)、N-acetyltyramine(4)、4-methyl-1-phenylpentane-2,3-diol(5)、1H-indole-3-aldehyde(6)以及zarzissine(7),其中化合物3和4的杀线虫活性较好.Study of the active material basis of killing nematodes by marine actinomycetes.Theliquid fermentation of eighty-one strains of marine Actinomycetes were screened for killing nematode,and then the fermentation liquid of the active strains was separated and purified,furthermore the compounds were screened for nematoidal activity.A marine Actinomycete strain with high nematicidal activity was screened and its nematode mortality rate reached 89.9%.The strain was identified as Streptomyces avidinii,seven compounds were obtained from its fermentation broth,and the compounds were named as pyrrole-2-carboxamide(1),N-(3-(1H-indole-3-yl)propyl)acetamide(2),p-hydroxybenzyl alcohol(3),N-acetyltyramine(4),4-methyl-1-phenylpentane-2,3-diol(5),1H-indole-3-aldehyde(6)and zarzissine(7).Among them,compounds 3 and 4 showed better nematicidal activity.

关 键 词：线虫 海洋放线菌 次生代谢产物 发酵液 

分 类 号：Q939[生物学—微生物学]