HPLC波长切换法测定除脂生发片4种中成药成分含量  

Determination of 4 proprietary Chinese medicines in Lipophilic Shengfa tablets by HPLC wavelength switching method

作　　者：边瑜 李秀春 Bian Yu;Li Xiuchun(Huainan Food and Drug Inspection Center,232000;Lu'an Food and Drug Inspection Center,237001)

机构地区：[1]淮南市食品药品检验中心,232000 [2]六安市食品药品检验中心,237001

出　　处：《现代科学仪器》2024年第3期51-55,共5页Modern Scientific Instruments

摘　　要：目的:建立了HPLC波长切换法测定除脂生发片中成药胡薄荷酮等4种成分含量的方法。方法:色谱柱:Agilent Eclipse Plus C18(4.6×250mm,5μm);以乙腈(A)-0.3%磷酸(B)为流动相,梯度洗脱,洗脱程序为:0~6min 89%A,6~27min 89%~31%A,27~30min 31%~89%A,31~35min 89%A;柱温30℃;柱流量1.0mL/min;检测波长:丹皮酚(274nm)、黄柏酮(236nm)、5-O-甲基维斯阿米醇苷(254nm)和胡薄荷酮(252nm);进样体积:10μL,HPLC-DAD定量检测。结果:丹皮酚、黄柏酮、5-O-甲基维斯阿米醇苷和胡薄荷酮分别在浓度范围0.01996~1.996、0.5868~5.868、0.997~9.97、0.0998~9.98μg/mL内具有良好的线性,平均回收率分别为100.92%、100.54%、101.14%、100.93%;精密度和重复性RSD均在5.0%以内。结论:此方法具有前处理简单等优点,适用于除脂生发片制剂的质量控制。Objective:A HPLC wavelength switching method was established for the determination of four components in Chuzhi Shengfa Tablets,including Humidone.Method:Agilent Eclipse Plus C18(4.6×250 mm,5μm)was used.The mobile phase was consisted of acetonitrile(A)-0.3%phosphoric acid solution(B)with gradient elution.The elution procedures were:0~6 min 89%A,6~27 min 89%~31%A,27~30 min 31%~89%A,31~35 min 89%A.Column temperature:30°C,column flow:1.0 mL/min.Detection wavelengths of paeonol,xibutanone,5-O-methylvisamitol side and humenthone were set at 274 nm、236 nm、254 nm and 252 nm,respectively.Injection volume was 10μL.HPLC-DAD for quantitative detection Result:Paeonol,obacunone,5-O-methylvisamitol side and pulegone showed good linear relationships in concentration range of 0.01996~1.996、0.5868~5.868、0.997~9.97、0.0998~9.98μg/mL.The average recoveries were 100.92%、100.54%、101.14%、100.93%,respectively.Precision and reproducibility of the method were within 5.0%.Conclusion:This method has the advantages of simple pretreatment,and is suitable for the quality control of lipophaga Shengfa tablets.

关 键 词：HPLC 除脂生发片 丹皮酚 黄柏酮 胡薄荷酮 

分 类 号：R28[医药卫生—中药学]