体外诱导间充质干细胞向成纤维细胞分化的研究  

Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Fibroblasts in vitro

作　　者：曾文 尹贝立 谭文松[1] 蔡海波[1] ZENG Wen;YIN Beili;TAN Wensong;CAI Haibo(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China;Guangdong Renrenkang Pharmaceutical Co.LTD,Zhuhai 519000,Guangdong,China)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]广东人人康药业有限公司,广东珠海519000

出　　处：《华东理工大学学报（自然科学版）》2024年第3期383-390,共8页Journal of East China University of Science and Technology

摘　　要：成纤维细胞(Fibroblast,FB)在美容医学和再生医学中展现出前所未有的潜力,然而成纤维细胞的获取以及数量有限是限制其临床应用的瓶颈。使用实验室自主设计的成纤维细胞诱导分化培养基DFM诱导间充质干细胞(Mesenchymal Stem Cells, MSCs)向成纤维细胞分化,并以诱导后的总细胞扩增倍数、成纤维细胞相关基因和蛋白的表达以及细胞的迁移能力为指标,建立了一个全面的分化效果评价体系。结果表明:成纤维细胞诱导分化培养基DFM中细胞的累计扩增倍数显著高于对照组;成纤维细胞相关基因和蛋白的表达显著高于对照组;细胞迁移能力也较对照组有显著提升。Fibroblast(FB)has shown unprecedented potential in cosmetic and regenerative medicine.However,the limited availability and quantity of fibroblasts are bottlenecks limiting their clinical application.In this study,we use DFM,a fibroblast-induced differentiation medium designed by our laboratory,to induce the differentiation of Mesenchymal Stem Cells(MSCs)into fibroblasts in vitro.The cell proliferation characteristics after induction are determined by cell counting,the expression of fibroblast-related genes is detected using qRT-PCR,and the expression of fibroblast-related proteins is measured by flow cytometry.In addition,the migration ability of induced cells is determined by scratch tests.Based on these data,a comprehensive evaluation system for cell differentiation efficiency is established.The results show that the expansion folds of total cells in the induction medium DFM are significantly higher than those in the control group;The expression of fibroblast-related genes such as VIM(vimentin),LN(laminin),and FSP-1(fibroblast-specific protein 1),as well as the expression of fibroblast-related proteins(including VIM,LN,and FSP-1)are significantly up-regulated in comparison with those in the control group.The migration ability of induced cells is also significantly enhanced in comparison with the control group.

关 键 词：成纤维细胞 间充质干细胞 培养基 分化 细胞迁移 

分 类 号：Q813.11[生物学—生物工程]