pH响应型抗菌水凝胶伤口敷料的制备及促愈合性能  

Preparation and Healing Promoting Properties of pH Responsive Antibacterial HydrogelWound Dressing

作　　者：尹祖秀 黄婷婷 王建英[2] 祁永浩 解莹莹 王光 刘会茹[1] 王娟[1] Zuxiu Yin;Tingting Huang;Jianying Wang;Yonghao Qi;Yingying Xie;Guang Wang;Huiru Liu;Juan Wang(Technology Innovation Center of Hebei for Fiber Material,Shijiazhuang University,Shijiazhuang 050035,China;School of Chemical and Pharmaceutical Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China)

机构地区：[1]石家庄学院河北省纤维材料技术创新中心,河北石家庄050035 [2]河北科技大学化学与制药工程学院,河北石家庄050018

出　　处：《高分子材料科学与工程》2024年第4期29-39,共11页Polymer Materials Science & Engineering

基　　金：石家庄市重点研发计划(231490112A)。

摘　　要：文中利用衣康酸(IA)、丙烯酰胺(AM)和烯丙基三甲基氯化铵(TAAC)为原料发生进行交联,负载抗菌药物聚六亚甲基盐酸胍(PHMG),一锅法制备了pH敏感性抗菌水凝胶IA/AM/TAAC@PHMG。对水凝胶的力学性能、溶胀性能、释药性能、抗菌性能、生物相容性及促感染伤口愈合性能进行了研究。结果表明,TAAC能增加水凝胶的柔韧性,使水凝胶的断裂伸长率提高了88%;水凝胶具有pH响应性,能积极响应伤口pH,控制和延长PHMG的释放,PHMG在碱性条件下(pH 8.2)的释放率(60.65%)优于酸性条件(pH 3.2)下的释放率(14.97%);IA/AM/TAAC@PHMG对大肠杆菌和金黄色葡萄球菌的抑菌率大于99.98%;水凝胶组(320μg/mL)的细胞相对存活率高于75.08%,细胞溶血率小于4%,抗菌水凝胶具有良好的生物相容性;组织学分析证明IA/AM/TAAC@PHMG载药水凝胶能通过创造湿润低氧利于肉芽生长的环境和优异的抗菌性能,使炎性因子减少,促进胶原和皮肤再生,加速伤口愈合。Bacterial infections can cause wound inflammation,make wound healing difficult,and there is an urgent need to develop intelligent hydrogel dressings that are highly effective in their antibacterial,anti-inflammatory properties,and can promote re-epithelialization and angiogenesis.The pH sensitive antibacterial hydrogel IA/AM/TAAC@PHMG was prepared by one pot method using itaconic acid(IA),acrylamide(AM)and allyl trimethylammonium chloride(TAAC)as raw materials for covalent cross-linking,loaded with the antibacterial drug polyhexamethylene guanidine hydrochloride(PHMG).The mechanical properties,swelling properties,drug release properties,antimicrobial properties,biocompatibility,and pro-infectious wound healing properties were tested.The results show that TAAC increases the flexibility of hydrogels and the breaking elongation of hydrogels is increased by 88%;the hydrogels are pH-responsive,can respond positively to wound,pH,control and prolong the release of PHMG,the release rate of PHMG under alkaline conditions(pH 8.2)(60.65%)is better than 14.97%under acidic conditions(pH 3.2);the antibacterial rate of IA/AM/TAAC@PHMG against E.coli and S.aureus is over 99.98%;the cell relative survival rate in the hydrogel group(320μg/mL)is above 75.08%,the cell hemolysis rate is below 4%,the antibacterial hydrogel has a good biocompatibility;histological analysis prove that the IA/AM/TAAC@PHMG loaded hydrogel can create an environment conducive to granulation growth and excellent antibacterial properties,to reduce the inflammatory factors,promote both collagen and skin regeneration,accelerate wound healing.

关 键 词：水凝胶 PH敏感性 药物释放 聚六亚甲基盐酸胍 抗菌性能 

分 类 号：TQ427.26[化学工程]