银杏叶提取物调控PI3K/AKT信号通路对人脑血管外膜成纤维细胞增殖和凋亡的影响  

Effects of Ginkgo Biloba extract regulating PI3K/AKT signaling pathway on proliferation and apoptosis of human cerebral vascular adventitia fibroblasts

作　　者：毕晓晨 孔晓彬 赵娜 顾晶晶[2] BI Xiao-chen;KONG Xiao-bin;ZHAO Na;GU Jing-jing(Experimental Teaching Center,School of Basic Medicine,Zhejiang Chinese Medicine University,Hangzhou 310000,Zhejiang Province,China;Department of Pathology and Pathophysiology,School of Basic Medicine,Zhejiang Chinese Medicine University,Hangzhou 310000,Zhejiang Province,China)

机构地区：[1]浙江中医药大学基础医学院实验教学中心,浙江杭州310000 [2]浙江中医药大学基础医学院病理与病理生理教研室,浙江杭州310000

出　　处：《中国临床药理学杂志》2024年第11期1556-1559,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金青年科学基金资助项目(81603487)。

摘　　要：目的研究银杏叶提取物(GBE)调控磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(AKT)信号通路对人脑血管外膜成纤维细胞(HBVAFs)增殖和凋亡的影响。方法将HBVAFs分为空白组(正常培养,未做处理)和低、中、高剂量实验组(25、50和100 mg·L^(-1)的GBE处理24 h)及联合组(100 mg·L^(-1)GBE和2 nmol·L^(-1)胰岛素样生长因子-1处理24 h)。用噻唑蓝法检测细胞的增殖情况,用流式细胞仪检测细胞的凋亡情况,用蛋白质印迹法检测细胞磷酸化PI3K(p-PI3K)、PI3K、磷酸化AKT(p-AKT)及AKT蛋白的表达水平。结果低、中、高剂量实验组和联合组、空白组的细胞存活率分别为(66.78±8.21)%、(53.51±7.40)%、(42.33±6.01)%、(75.69±5.87)%和(96.77±12.80)%,细胞凋亡率分别为(24.83±2.47)%、(30.07±3.06)%、(37.02±4.66)%、(16.71±2.11)%和(8.99±0.19)%,p-PI3K/PI3K比值分别为0.75±0.07、0.62±0.06、0.43±0.04、0.72±0.05和0.90±0.09,p-AKT/AKT比值分别为0.70±0.07、0.60±0.06、0.38±0.03、0.69±0.06和0.90±0.09。低、中、高剂量实验组的上述指标与空白组比较,联合组的上述指标与高剂量实验组比较,在统计学上差异均有统计学意义(均P<0.05)。结论GBE可降低HBVAFs增殖,促进凋亡,其机制与抑制PI3K/AKT信号通路相关。Objective To study the effects of Ginkgo biloba extract(GBE)on the proliferation and apoptosis of human cerebral adventitia fibroblasts(HBVAFs)by regulating the phosphatidylinositol 3 kinase(PI3K)/protein kinase B(AKT)signaling pathway.Methods HBVAFs cells were divided into blank group(normal culture,no treatment),experimental-L,-M,-H groups(25,50 and 100 mg·L^(-1)GBE treatment for 24 h)and combined group(100 mg·L^(-1)GBE treatment and 2 nmol·L^(-1)insulin-like growth factor-1 treatment for 24 h).Cell proliferation was detected by thiazole blue assay,cell apoptosis was detected by flow cytometry,and the protein expressions of phosphorylated-PI3K(p-PI3K),PI3K、phosphorylated-AKT(p-AKT)and AKT were detected by Western blot.Results The cell survival rates of experimental-L,experimental-M,experimental-H,combined and blank groups were(66.78±8.21)%,(53.51±7.40)%(42.33±6.01)%,(75.69±5.87)%and(96.77±12.80)%;the apoptosis rates were(24.83±2.47)%,(30.07±3.06)%,(37.02±4.66)%,(16.71±2.11)%and(8.99±0.19)%;p-PI3K/PI3K ratios were0.75±0.07,0.62±0.06,0.43±0.04,0.72±0.05 and 0.90±0.09;p-AKT/AKT ratios were 0.70±0.07,0.60±0.06,0.38±0.03,0.69±0.06 and 0.90±0.09,respectively.The above indexes of the experimental-L,-M,-H groups were compared with the blank group,and the above indexes of the combined group were compared with theexperimental-H group,the differences were statistically significant(allP<0.05).Conclusion GBE can reduce theproliferation of HBVAFs,promote apoptosis,and its mechanism is related to the inhibition of PI3K/AKT signaling pathway.

关 键 词：银杏叶提取物 磷酸肌醇3激酶/蛋白激酶B信号通路 人脑血管外膜成纤维细胞 增殖 凋亡 

分 类 号：R28[医药卫生—中药学]