环状RNA_0054633对过氧化氢诱导的心肌细胞氧化损伤的影响  

Effects of circular RNA_0054633 on hydrogen peroxide-induced oxidative damage in myocardial cells

作　　者：王艳炜 吴变稳 曹磊 马杰 封亚丽[3,4] WANG Yan-wei;WU Bian-wen;CAO Lei;MA Jie;FENG Ya-li(Department of Second Cardiology,The First Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei Province,China;Department of Cardiovascular Medicine,980th Hospital of The Joint Logistics Support Force of The People's Liberation Army of China,,Shijiazhuang 050082,Hebei Province,China;Second Department of Cardiovascular Medicine,Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang 050031,Hebei Province,China;Key Laboratory of Turbidity and Toxicity Syndrome in Hebei Province,Shijiazhuang 050031,Hebei Province,China)

机构地区：[1]河北医科大学第一医院心脏外二科,河北石家庄050000 [2]中国人民解放军联勤保障部队第980医院心血管内科,河北石家庄050082 [3]河北省中医院心血管内二科,河北石家庄050031 [4]河北省浊毒证重点实验室,河北石家庄050091

出　　处：《中国临床药理学杂志》2024年第11期1560-1564,共5页The Chinese Journal of Clinical Pharmacology

基　　金：河北省中医药管理局科研计划基金资助项目(2018047);河北中医学院教育教学改革研究项目立项课题基金资助项目(22yb-16)。

摘　　要：目的考察环状RNA(circRNA)circ_0054633是否靶向miR-375调控过氧化氢(H_(2)O_(2))诱导的心肌细胞氧化损伤。方法将H9C2心肌细胞分为对照组(正常培养,未进行任何处理)、H_(2)O_(2)组(150μmol·L^(-1)H_(2)O_(2)处理心肌细胞6 h)、H_(2)O_(2)+si-NC组、H_(2)O_(2)+si-circ_0054633组、H_(2)O_(2)+miR-NC组、H_(2)O_(2)+miR-375组、H_(2)O_(2)+si-circ_0054633+anti-miR-NC组、H_(2)O_(2)+si-circ_0054633+anti-miR-375组(在心肌细胞中分别转染si-NC、si-circ_0054633、miR-NC、miR-375模拟物、si-circ_0054633+anti-miR-NC、si-circ_0054633+anti-miR-375,转染24 h后,用150μmol·L^(-1)H_(2)O_(2)处理心肌细胞6 h)。用实时荧光定量聚合酶链反应法测定miR-375的表达水平,用试剂盒测定丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,用流式细胞术测定细胞凋亡情况。结果对照组、H_(2)O_(2)组、H_(2)O_(2)+si-NC组、H_(2)O_(2)+si-circ_0054633组、H_(2)O_(2)+miR-NC组、H_(2)O_(2)+miR-375组、H_(2)O_(2)+si-circ_0054633+anti-miR-NC组、H_(2)O_(2)+si-circ_0054633+anti-miR-375组的心肌细胞miR-375表达量分别为1.00±0.00、0.42±0.05、0.40±0.06、0.69±0.08、1.00±0.00、3.41±0.28、1.00±0.00和0.26±0.02,MDA含量分别为(3.19±0.32)、(13.46±1.27)、(15.39±1.04)、(5.26±0.51)、(16.05±1.36)、(9.18±0.85)、(4.89±0.44)和(10.05±0.92)nmol·L^(-1),SOD活性分别为(64.69±5.81)、(18.23±1.33)、(17.07±1.41)、(55.74±5.13)、(17.12±1.64)、(47.66±4.59)、(56.77±4.71)和(27.95±2.47)U·mL^(-1),凋亡率分别为(6.21±0.59)%、(29.22±2.19)%、(30.94±2.85)%、(10.05±0.92)%、(31.14±2.83)%、(13.74±1.24)%、(10.39±0.88)%和(21.31±1.92)%。H_(2)O_(2)组的上述指标与对照组比较,H_(2)O_(2)+si-circ_0054633组的上述指标与H_(2)O_(2)+si-NC组比较,H_(2)O_(2)+miR-375组的上述指标与H_(2)O_(2)+miR-NC组比较,H_(2)O_(2)+si-circ_0054633+anti-miR-375组的上述指标与H_(2)O_(2)+si-circ_0054633+anti-miR-NC组比较,在统计学上差异均有统计学意义(均P<0.05)。结论干扰circObjective To investigate whether circular RNA(circRNA)circ_0054633 targets microRNA-375(miR-375)to regulate the oxidative damage of cardiomyocytes induced by hydrogen peroxide(H_(2)O_(2)).Methods H9C2 cardiomyocytes were divided into control group(normal culture without any treatment),hydrogen peroxide(H_(2)O_(2))group(150μmol·L^(-1)H_(2)O_(2)treatedcardiomyocytes for 6 h),H_(2)O_(2)+si-NC group,H_(2)O_(2)+si-circ_0054633 group,H_(2)O_(2)+miR-NC group,H_(2)O_(2)+miR-375 group,H_(2)O_(2)+si-circ_0054633+anti-miR-NC group,H_(2)O_(2)+si-circ_0054633+anti-miR-375group(cardiomyocytes were transfected withsi-NC,si-circ_0054633,miR-NC,miR-375mimics,si-circ_0054633+anti-miR-NC,si-circ_0054633+anti-miR-375,respectively;24 h after transfection,cardiomyocytes were treated with 150μmol·L^(-1)H2O for 6 h).Real-time fluorescence quantitative polymerase chainreaction was used to determine the expression ofmiR-375,the content of malondialdehyde(MDA)and the activity ofsuperoxide dismutase(SOD)were determined by kit,apoptosis was determined by flow cytometry.Results Theexpression levels ofmiR-375 in cardiomyocytes of control group,H_(2)O_(2)group,H_(2)O_(2)+si-NC group,H_(2)O_(2)+si-circ_0054633 group,H_(2)O_(2)+miR-NC group,H_(2)O_(2)+si-circ_0054633+anti-miR-NC group,H_(2)O_(2)+si-circ_0054633+anti-miR-375 group were 1.00±0.00,0.42±0.05,0.40±0.06,0.69±0.08,1.00±0.00,3.41±0.28,1.00±0.00 and 0.26±0.02,respectively;MDA contents were(3.19±0.32),(13.46±1.27),(15.39±1.04),(5.26±0.51),(16.05±1.36),(9.18±0.85),(4.89±0.44)and(10.05±0.92)nmol·L^(-1),respectively;SOD activities were(64.69±5.81),(18.23±1.33),(17.07±1.41),(55.74±5.13),(17.12±1.64),(47.66±4.59),(56.77±4.71)and(27.95±2.47)U·mL^(-1),respectively;the apoptosis rateswere(6.21±0.59)%,(29.22±2.19)%,(30.94±2.85)%,(10.05±0.92)%,(31.14±2.83)%,(13.74±1.24)%,(10.39±0.88)%and(21.31±1.92)%,respectively.The above indexes of H_(2)O_(2)group were compared with thecontrol group,the above indexes of H_(2)O_(2)+si-circ_0054633 group was compared with the H_

关 键 词：环状RNA_0054633 微小RNA-375 过氧化氢 心肌细胞 氧化应激 凋亡 

分 类 号：R97[医药卫生—药品]