山姜素调控PI3K/Akt/NF-κB信号通路对冠心病大鼠心肌损伤的影响  

Effects of PI3K/Akt/NF-κB signaling pathway regulated by lozingin on myocardial injury in rats with coronary heart disease

作　　者：刘裕 左清平 何鸽飞 严建业[1] LIU Yu;ZUO Qingping;HE Gefei(Science and Technology Innovation Center of Hunan University of Traditional Chinese Medicine,Changsha Hunan 410208,China;Clinical Trial Research Center of Changsha First Hospital,Changsha Hunan 410005,China;Pharmacy Department of Changsha First Hospital,Changsha Hunan 410005,China.)

机构地区：[1]湖南中医药大学科技创新中心,湖南长沙410208 [2]长沙市第一医院临床试验研究中心,湖南长沙410005 [3]长沙市第一医院药剂科,湖南长沙410005

出　　处：《四川中医》2024年第5期79-82,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：湖南省自然科学基金项目(编号:2021JJ40619);长沙市自然科学基金项目(编号:KQ2202013)。

摘　　要：目的:探讨山姜素调控磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)/核转录因子-κB(NF-κB)信号通路对冠心病(CHD)大鼠心肌损伤的影响。方法:选取健康成年SPF级SD雄性大鼠60只,随机分为空白组、模型组、山姜素小剂量组、山姜素中剂量组、山姜素高剂量组各12只,采用Western blot法检测大鼠PI3K/Akt/NF-κB信号通路蛋白表达,采用ELISA法检测大鼠炎症因子指标[肿瘤坏死因子α(TNF-α)、白介素-1β(IL-1β)、白介素-18(IL-18)]和氧化应激指标[活性氧(ROS)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)],观察并记录大鼠心肌损伤情况。结果:与空白组比较,模型组的心肌缺血和心肌梗死面积更大(P<0.05),与模型组比较,山姜素小、中、高剂量组的心肌缺血和心肌梗死面积均缩小(P<0.05),且高剂量组的心肌缺血和心肌梗死面积小于山姜素中、小剂量组,中剂量组心肌缺血和心肌梗死面积小于山姜素小剂量组(P<0.05)。与空白组比较,模型组的PI3K、Akt、NF-κB更高(P<0.05),与模型组比较,山姜素小、中、高剂量组的PI3K、Akt、NF-κB均升高(P<0.05),且高剂量组的PI3K、Akt、NF-κB高于山姜素中、小剂量组,中剂量组PI3K、Akt、NF-κB高于山姜素小剂量组(P<0.05)。与空白组比较,模型组的TNF-α、IL-1β、IL-18更高(P<0.05),与模型组比较,山姜素小、中、高剂量组的TNF-α、IL-1β、IL-18均降低(P<0.05),且山姜素高剂量组的TNF-α、IL-1β、IL-18低于中、小剂量组,山姜素中剂量组的TNF-α、IL-1β、IL-18低于山姜素小剂量组(P<0.05)。与空白组比较,模型组的ROS、GSH-Px、MDA更高(P<0.05),与模型组比较,山姜素小、中、高剂量组的ROS、GSH-Px、MDA均降低(P<0.05),且山姜素高剂量组ROS、GSH-Px、MDA低于山姜素中、小剂量组,山姜素中剂量组的ROS、GSH-Px、MDA低于山姜素小剂量组(P<0.05)。结论:山姜素可通过调控PI3K/Akt/NF-κB信号通路改善CHD大鼠心肌炎症和氧化应�Objective To investigate the effects of alpinogenin regulating phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/nuclear transcription factor-κB(NF-κB)signaling pathway on myocardial injury in rats with coronary heart disease(CHD).Methods 60healthy adult SD rats with SPF were randomly divided into blank group,model group,small dose group,medium dose group and high dose group with 12rats each.The PI3K/Akt/NF-κB signaling pathway protein expression was detected by Western blot assay.Inflammatory factors[tumor necrosis factorα(TNF-α),interleukin-1β(IL-1β),interleukin-18(IL-18)]and oxidative stress[reactive oxygen species(ROS),glutathione peroxidase(GSH-Px),malondialdehyde(MDA)]were detected by ELISA.The myocardial injury of rats was observed and recorded.Results Compared with the blank group,the area of myocardial ischemia and myocardial infarction in the model group was larger(P<0.05).Compared with the model group,the area of myocardial ischemia and myocardial infarction in the small,medium and high dose groups of lozingin decreased(P<0.05),and the area of myocardial ischemia and myocardial infarction in the high dose group was smaller than that in the medium and small dose groups of lozingin.The area of myocardial ischemia and myocardial infarction in medium dose group was smaller than that in small dose group(P<0.05).Compared with blank group,PI3K,Akt and NF-κB in model group were higher(P<0.05),and PI3K,Akt and NF-κB in small,medium and high dose groups were increased compared with model group(P<0.05),and PI3K,Akt and NF-κB in high dose group were higher than those in medium and low dose groups.PI3K,Akt and NF-κB in medium dose group were higher than those in small dose group(P<0.05).Compared with the blank group,the levels of TNF-α,IL-1β and IL-18in the model group were higher(P<0.05),and the levels of TNF-α,IL-1βand IL-18in the small,medium and high dose groups were lower than those in the model group(P<0.05).The levels of TNF-α,IL-1β and IL-18in high-dose group were lower than those i

关 键 词：山姜素 磷脂酰肌醇3-激酶 蛋白激酶B 核转录因子-ΚB 冠心病 心肌损伤 

分 类 号：R285.5[医药卫生—中药学]