苗药黑骨藤对CIA大鼠外周血Th22细胞数量及血清IL-17、IL-22和IL-35表达水平的影响  

The effect of Miao medicine Periploca forrestii on the number of Th22 cells and the expression levels of IL-17,IL-22,and IL-35 in CIA rats

作　　者：罗康华[1] 王恒[1] 梁子聪[1] 罗静 蒋催蓉[1] Luo Kanghua;Wang Heng;Liang Zicong;Luo Jing;Dang Rongmin;Jiang Chaorong(Qiannan Medical College For Nationalities,Duyun 558000 Guizhou;The Affillated Hospital Of Guizhou Medical University,Guiyang 550004 Guizhou)

机构地区：[1]黔南民族医学高等专科学校,贵州都匀558000 [2]贵州医科大学附属医院病理科,贵州贵阳550004

出　　处：《中兽医学杂志》2024年第4期4-6,共3页Chinese Journal of Traditional Veterinary Science

基　　金：黔南科技计划项目(编号:黔南科合社字[2022]4号);黔南民族医学高等专科学校科研基金资助项目(编号:QNYZ201709)。

摘　　要：目的观察苗药黑骨藤对CIA大鼠外周血Th22细胞数量和细胞因子IL-17、IL-22和IL-35的表达水平的影响。将60只CIA大鼠作为研究对象,按随机数字表法随机分为正常对照组、模型对照组、阳性对照组(雷公藤多苷,9.45mg/kg)、黑骨藤低剂量组(22 mg/kg)、中剂量组(44 mg/kg)和高剂量组(67 mg/kg),每组10只。建立大鼠CIA模型,连续给药21 d后测定大鼠血清IL-17、IL-22、IL-35水平和Th22细胞数量,观察大鼠足趾组织镜下表现。结果与模型对照组比较,阳性对照组、黑骨藤低剂量组、剂量组血清、高剂量组血清IL-17、IL-22水平以及Th22细胞比例明显降低,血清IL-35水平明显增高,黑骨藤高剂量组在降低血清IL-17、IL-22水平,升高血清IL-35水平效果上优于阳性对照组。结论黑骨藤可以减少Th22细胞数量,调节IL-17、IL-22、IL-35等细胞因子表达,具有减少炎症细胞活化和浸润、抑制关节炎症的作用,从而减缓类风湿关节炎的进展。Objective To observe the effects of Miao medicine Periploca forrestii on the number of Th22 cells and the expression levels of IL-17,IL-22,and IL-35 in CIA rats.Methods Sixty CIA rats were randomLy divided into normal control group,model control group,positive control group(Tripterygium wilfordii glycosides tablets,9.45 mg/kg),Periploca forrestii low-dose group(22 mg/kg),medium-dose group(44 mg/kg),and high-dose group(67 mg/kg),with 10 rats in each group.The CIA model in rats was established and given medication for 21 consecutive days.The serum levels of IL-17,IL-22,and IL-35 and the number of Th22 cells were measured,and the performance of foot tissue under microscopy was observed.Results Compared with the model control group,the positive control group,Periploca forrestii low-dose group,medium-dose group,and high-dose group showed a significant decrease in the levels of IL-17,IL-22,and the proportion of Th22 cells,while the level of IL-35 in serum significantly increased.The high-dose group of Periploca forrestii was superior to the positive control group in reducing the levels of IL-17,IL-22 in serum and increasing the level of IL-35.Conclusion Periploca forrestii can reduce the number of Th22 cells,regulate the expression of cytokines such as IL-17,IL-22,and IL-35,play a role in reducing the activation and infiltration of inflammatory cells,inhibiting joint inflammation,thereby slowing down the progression of RA.

关 键 词：黑骨藤 类风湿关节炎 TH22细胞 

分 类 号：R285.5[医药卫生—中药学]