羊GV期卵母细胞玻璃化冷冻保存研究进展  

Advances in Vitrification Cryopreservation of Ovine GV Oocytes

作　　者：张迪 房晓欢 李俊杰[1,2] 王志刚 ZHANG Di;FANG Xiaohuan;LI Junjie;WANG Zhigang(College of Animal Science and Technology,Hebei Agricultural University,Baoding,Hebei 07100;Hebei Technology Innovation Center of Cattle and Sheep Embryo,Baoding,Hebei 071000,China)

机构地区：[1]河北农业大学动物科技学院,河北保定071000 [2]河北省牛羊胚胎技术创新中心,河北保定071000

出　　处：《家畜生态学报》2024年第6期9-13,共5页Journal of Domestic Animal Ecology

基　　金：“十四五”国家重点研发计划项目(2021YFD1200401);河北省科技计划(17226613D)。

摘　　要：生发泡(germinal vesial,GV)期卵母细胞由于未形成极为敏感的纺锤体,对于冷冻卵母细胞以及长期保存地方家畜种质资源具有无可比拟的优势。研究人员对此进行了广泛的关注,基于此,本文综述了羊GV期卵母细胞玻璃化冷冻保存的意义,分析了目前羊GV期卵母细胞冷冻保存过程中存在的问题,总结了有效提高卵母细胞冷冻效率的一些解决方法,对提高羊GV期卵母细胞冷冻保存效率具有一定的借鉴与参考。Oocytes vitrification at germinal vesial(GV)stage has unrivaled advantages for freezing oocytes and long-term preservation of local livestock germplasm resources because they do not form the extremely sensitive spindle,which have attracted researchers'wide attention.On the basis of it,this paper reviews the significance of oocyte vitrification cryopreservation of sheep at GV stage.It analyzes the problems in current oocyte cryopreservation process of sheep at GV stage,and summarizes the effective solutions to improve the oocyte freezing efficiency.It provides reference for improving the efficiency of oocyte cryopreservation in the GV stage of sheep and goat.

关 键 词：绵羊 山羊 卵母细胞 GV期 玻璃化冷冻 

分 类 号：S826.3[农业科学—畜牧学]