西洋参茎叶皂苷对力竭运动大鼠心肌损伤的改善作用  

Effect and Mechanism of Panax quinnifolium Saponins onMyocardial Injury in Rats after Exhausted Exercise

作　　者：蓝瑞高 梁益军 LAN Ruigao;LIANG Yijun(Sports Department,Guilin University of Electronic Science and Technology,Guilin,Guangxi 540004,China)

机构地区：[1]桂林电子科技大学体育部,广西桂林540004

出　　处：《家畜生态学报》2024年第6期29-36,共8页Journal of Domestic Animal Ecology

基　　金：新世纪广西高等教育教学改革工程项目(2014JGB140)。

摘　　要：为了研究西洋参茎叶皂苷减轻力竭运动大鼠心肌损伤的作用及机制,为西洋参茎叶皂苷的综合利用提供参考,本试验分为正常对照组、力竭运动组及西洋参茎叶皂苷低、中、高剂量组(100、200、400 mg/kg),除正常对照组外均需进行力竭游泳训练,4周后检测相关指标变化。结果表明,(1)与力竭运动组相比,西洋参茎叶皂苷中、高剂量组大鼠LVIDs、LVIDd极显著减少(P<0.01),低、中、高剂量组LVEF、LVFS极显著增加(P<0.01),心肌组织cTnⅠ含量及血清CTNK、LDH、ALT、α-HBDH活性均极显著降低(P<0.01);中、高剂量组LVIDs、LVIDd同低剂量组比极显著减少(P<0.01),高剂量组LVEF极显著高于低、中剂量组(P<0.01),中、高剂量组LVFS极显著高于低剂量组(P<0.01),而CTNK、α-HBDH活性极显著低于低剂量组(P<0.01),高剂量组LDH活性极显著低于低、中剂量组(P<0.01),低、中、高剂量组ALT活性比较差异均有极显著性(P<0.01)。(2)与力竭运动组相比,西洋参茎叶皂苷低、中、高剂量组大鼠心肌组织及血清MDA含量极显著降低(P<0.01),除低剂量组大鼠血清GSH-Px活性显著增加(P<0.05)外,其余各组大鼠心肌组织及血清SOD、CAT及GSH-Px活性均极显著增加(P<0.01);高剂量组心肌组织及血清MDA含量极显著低于低、中剂量组(P<0.01),SOD、CAT活性则极显著高于低、中剂量组(P<0.01),中、高剂量组GSH-Px活性极显著高于低剂量组(P<0.01)。(3)与力竭运动组相比,西洋参茎叶皂苷低剂量组大鼠心肌组织细胞凋亡率显著降低(P<0.05),中、高剂量组细胞凋亡率及各剂量组Bax、cleaved caspase-3表达极显著降低(P<0.01),而Bcl-2表达极显著增加(P<0.01);中、高剂量组细胞凋亡率、Bax及cleaved caspase-3表达均极显著少于低剂量组(P<0.01),而Bcl-2表达极显著高于低剂量组(P<0.01)。本研究显示,西洋参茎叶皂苷具有减轻力竭运动大鼠心肌损伤及改善心功能作用,且该作用与抑制氧化应�To study the effects and mechanism of Panax quinnifolium saponins(PQS)on myocardial injury in rats after exhausted exercise,and to provide reference for the comprehensive utilization of PQS,rats were divided into normal control group,exhausted exercise group and low-,medium-and high dose PQS groups(100,200,400 mg/kg).All rats underwent exhausted swimming training except for the normal control group,and the changes of related indexes were detected 4 weeks later.Results showed(1)compared with exhausted exercise group,LVIDs and LVIDd in medium-and high dose PQS groups were extremely decreased(P<0.01),LVEF and LVFS in low-,medium-and high dose PQS groups were extremely increased(P<0.01),the content of cTnⅠin myocardial tissue and the activities of CTNK,LDH,ALT andα-HBDH in serum in low-,medium-and high dose PQS groups were extremely decreased(P<0.01).LVIDs and LVIDd in medium-and high dose PQS groups were extremely lower than those in low dose group(P<0.01),LVEF in high dose PQS group was extremely higher than that in low-and medium dose group(P<0.01),LVFS in medium-and high dose PQS group were extremely higher than that in low dose group(P<0.01).The activities of CTNK andα-HBDH in medium-and high dose PQS groups were extremely lower than that in low dose group(P<0.01),the activity of LDH in high dose PQS group was extremely lower than that in low-and medium dose groups(P<0.01),and the activity of ALT in low-,medium-and high dose groups was significantly different(P<0.01).(2)Compared with exhausted exercise group,the MDA content in myocardial tissue and serum of low-,medium-and high dose PQS groups was significantly decreased(P<0.01),except that serum GSH-Px activity in low dose PQS group was significantly increased(P<0.05),the activities of SOD,CAT and GSH-Px in myocardial tissue and serum of other groups extremely increased(P<0.01).The MDA content in myocardial tissue and serum of high dose PQS group was significantly lower than those of low-and medium dose groups(P<0.01),the activities of SOD and CAT in high d

关 键 词：西洋参茎叶皂苷 力竭运动 氧化应激 心肌损伤 

分 类 号：S858.2[农业科学—临床兽医学] R285.5[农业科学—兽医学]