NaYF_(4):Yb^(3+),Er^(3+)@SiO_(2)荧光纳米传感器检测牛奶中过氧化氢  

Preparation of a Fluorescent Nanosensor Based on NaYF_(4)∶Yb^(3+),Er^(3+)@SiO_(2)for Detection of Hydrogen Peroxide in Milk

作　　者：彭孔浩 彭微 白安琪 王凌男 赵伟馨 武玥 郭文 李淑荣[1,2] 罗利霞 孟佩俊 PENG Kong-Hao;PENG Wei;BAI An-Qi;WANG Ling-Nan;ZHAO Wei-Xin;WU Yue;GUO Wen;LI Shu-Rong;LUO Li-Xia;MENG Pei-Jun(School of Public Health,Baotou Medical College,Baotou 014040,China;Inner Mongolia Autonomous Region Health Testing and Evaluation Engineering Technology Center,Baotou 014040,China)

机构地区：[1]包头医学院公共卫生学院,包头014040 [2]内蒙古自治区卫生检测与评价工程技术中心,包头014040

出　　处：《分析化学》2024年第5期685-694,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.81960601,82360656);内蒙古自治区高等学校青年科技英才支持项目(No.NJYT23026);内蒙古自治区自然科学基金项目(Nos.2023MS08009,2021MS08055);内蒙古自治区卫生健康委医疗卫生科技计划项目(No.202201383);包头医学院研究生科研创新项目(No.bycx2023006);2023年自治区级大学生创新创业训练计划项目(No.S202310130001)资助。

摘　　要：采用溶剂热法合成了稀土掺杂上转换纳米材料NaYF_(4):Yb^(3+),Er^(3+),使用反相微乳液法对其表面进行SiO_(2)包覆制得NaYF_(4):Yb^(3+),Er^(3+)@SiO_(2)。采用透射电镜、X射线衍射和傅里叶红外光谱对此材料进行了表征,并基于NaYF_(4):Yb^(3+),Er^(3+)@SiO_(2)与方酸铁(SQA-Fe^(3+))之间的荧光猝灭原理构建了NaYF_(4):Yb^(3+),Er^(3+)@SiO_(2)-SQA-Fe^(3+)荧光纳米传感器,用于微量过氧化氢(H_(2)O_(2))的检测。在最佳实验条件下,本方法检测H_(2)O_(2)的线性范围为1.8~84.0μmol/L,检出限(3σ)为0.47μmol/L,牛奶样品中H_(2)O_(2)的加标回收率为98.4%~99.7%。本方法具有检出限低、稳定性好以及抗干扰能力强等优点,可用于牛奶样品中H_(2)O_(2)残留的检测。The rare-earth-elements-doped upconversion nanoparticles NaYF_(4):Yb^(3+),Er^(3+)were synthesized by solvothermal method,and NaYF_(4):Yb^(3+),Er^(3+)@SiO_(2) were prepared by coating SiO_(2) on the surface of NaYF_(4):Yb^(3+),Er^(3+)by inverse microemulsion method in this work.Based on the fluorescence quenching principle between the NaYF_(4)∶Yb^(3+),Er^(3+)@SiO_(2) and SQA-Fe^(3+),a NaYF_(4)∶Yb^(3+),Er^(3+)@SiO_(2)-SQA-Fe^(3+)fluorescence nanosensor was constructed for detection of trace hydrogen peroxide(H_(2)O_(2)).Under optimal conditions,the linear range of this method for detecting H_(2)O_(2) was 1.8‒84.0μmol/L,with detection limit(3σ)of 0.47μmol/L.The recoveries of H_(2)O_(2) spiked in milk were 98.4%‒99.7%.This method could be used for detection of H_(2)O_(2) residue in milk samples,with advantages such as low detection limit,good stability and strong anti-interference ability.

关 键 词：稀土掺杂上转换纳米材料 过氧化氢 荧光纳米传感器 荧光猝灭 

分 类 号：O657.3[理学—分析化学] TS252.7[理学—化学]