针刺通过增加前额叶皮层突触蛋白的表达对两种抑郁症动物模型的快速抗抑郁作用  

The rapid antidepressant effect of acupuncture on two animal models of depression by increasing the expression of synaptic proteins in the prefrontal cortex

作　　者：宁百乐 王志芳[2] 杨晴 吴倩 邓启粤[1,2] 高静 傅文[3] 邓颖 吴炳鑫 黄熙畅[1,2] 王聪 符文彬[1,2] Bai-le NING;Zhi-fang WANG;Qing YANG;Qian WU;Qi-yue DENG;Jing GAO;Wen FU;Ying DENG;Bing-xin WU;Xi-chang HUANG;Cong WANG;Wen-bin FU(The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China;Guangzhou University of Chinese Medicine,Guangzhou 510006,China;The First Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450006,China)

机构地区：[1]广州中医药大学第二附属医院,广州510120 [2]广州中医药大学,广州510006 [3]河南中医药大学第一附属医院,郑州450006

出　　处：《World Journal of Acupuncture-Moxibustion》2024年第2期117-129,共13页世界针灸杂志（英文版）

基　　金：Supported by the National Natural Science Funds(82074522,U23A20507);Guangzhou Science and Technology Planning Project(202102010247);the Natural Science Foundation of Guangdong Province(2021A1515011464,2019A1515012043);Research Fund for Bajian Talents of Guangdong Provincial Hospital of Chinese Medicine(BJ2022KY10)。

摘　　要：Objective:To investigate the effect of acupuncture onset time and synaptic structure in two different models of depression.Methods:A total of 150 healthy male adult SPF C57BL/6J mice were divided into five time-point groups:1 h,3 h,6 h,12 h and 24 h.Each time-point group was further divided into three groups:the model,scopolamine and acupuncture groups with 10 rats each.Rats were given forced swimming stimulation(15 min,once)before the intervention.A total of 200 healthy male adult specific pathogen-free(SPF)Sprague-Dawley(SD)rats were divided into 30 normal rats,and 170 modelling ones.A total of 90 rats were successfully modeled,and were randomly divided into three time-point groups:1 h,6 h and 24 h.And each time-point group was further divided into the normal,model,scopolamine,and acupuncture group.Chronic unpredictable mild stress(CUMS)combined with individual feeding was used to establish the depressed rat model.The sucrose preference test(SPT)and open field test(OFT)were used to evaluate the success of the model.There were 10 rats in each group.The acupuncture intervention was performed once for 20 min on"Baihui(GV20)","Yintang(GV29)","Hegu(LI4)",and"Taichong(LR3)"without any other operations for the corresponding animals.Other groups were given corresponding medications.According to different time points,the forced swimming test(FST)was conducted in mice,and the FST and novelty-suppressed feeding test(NSFT)were measured in rats.Monoamine neurotransmitters(NE,DA,DOPAC,5-HT,5-HIAA,HVA)in the prefrontal cortex and hippocampus were detected with high performance liquid chromatography(HPLC).The expression of synapsin I,PSD95,p-mTOR,mTOR,and BDNF in the prefrontal cortex and hippocampus was determined by western blot.Results:At the 1 h time point,compared with the model group,the immobility time was significantly decreased in the acupuncture group(P<0.05).At 3 h,6 h,12 h,and 24 h,compared with the model group,the immobility time was significantly reduced in both scopolamine and acupuncture groups(P<0.05).At the 1

关 键 词：DEPRESSION ACUPUNCTURE Rapidantidepressanteffect Synaptic proteins Prefrontal cortex 

分 类 号：R245[医药卫生—针灸推拿学]