链带藻源生物刺激剂提高小麦对盐胁迫的生理适应  

Application of Desmodesmus sp.extract as a biostimulant improves physiological adaptation of wheat to salt stress

作　　者：谭梅娟 王捷 王清华 王嘉姝 余晨钶 刘琪[3] TAN Meijuan;WANG Jie;WANG Qinghua;WANG Jiashu;YU Chenke;LIU Qi(College of Biological Science and Technology,Taiyuan Normal University,Jinzhong,Shanxi 030619,China;Shanxi Key Laboratory of Earth Surface Processes and Resource Ecology Security in Fenhe River Basin,Taiyuan Normal University,Jinzhong,Shanxi 030619,China;College of Life Science,Shanxi University,Taiyuan 030006,China)

机构地区：[1]太原师范学院生物科学与技术学院,山西晋中030619 [2]太原师范学院汾河流域地表过程与资源生态安全山西省重点实验室,山西晋中030619 [3]山西大学生命科学学院,太原030006

出　　处：《西北植物学报》2024年第6期853-862,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(32000167,31970217);2023年山西省高等学校教学改革创新项目(J20231101);2023年山西省高等学校大学生创新创业训练项目(20230927)。

摘　　要：[目的]盐胁迫是影响小麦萌发、生长和生产的最重要环境因素。探究链带藻生物刺激剂对盐胁迫条件下小麦种子和早期幼苗抗盐、生长和生理的缓解效应以及最佳施用浓度,可为其应用于缓解小麦盐胁迫提供理论依据。[方法]通过室内培养皿培养法,将小麦种子置于100 mmol/L NaCl胁迫下,外源添加25,50,100,200 mg/L的链带藻提取物(DAE),处理7 d后测量各项萌发和生长参数。[结果]外源添加DAE处理缓解了盐胁迫对小麦种子萌发和早期幼苗生长的抑制作用,提高了盐胁迫下小麦种子的萌发率和叶片含水量,促进了生物量的积累;提高了幼苗叶片超氧化物歧化酶、过氧化物酶、过氧化氢酶、抗坏血酸过氧化物酶活性以及脯氨酸、可溶性总糖、可溶性蛋白质和叶绿素的含量;降低了脂质过氧化作用,减少了丙二醛含量和膜透性。在100 mmol/L NaCl胁迫条件下,25 mg/L DAE对盐胁迫下小麦种子萌发及早期幼苗生长的促进作用效果最佳。[结论]链带藻细胞提取物通过促进小麦种子早期萌发的启动,提高小麦幼苗叶绿素含量、抗氧化酶活性和渗透调节能力,增强小麦种子及早期幼苗对盐胁迫的适应性,提升了小麦的耐盐能力。[Objective]Salt stress is the most important environmental factor affecting wheat germination,growth,and production.In order to investigate the alleviation effect of the biological stimulator Desmodesmus sp.on growth and physiology of wheat seeds and early seedlings under salt stress as well as screening the best application concentration,the study can provide a basis for its application to alleviate the impact of salt stress on wheat.[Methods]Using the petri dish culture method,wheat seeds germinated under 100 mmol/L NaCl were treated with 25,50,100,and 200 mg/L of Desmodesmus sp.extracts(DAE),and germination and growth parameters were measured after 7 days.[Results]DAE alleviated the inhibitory effect of salt stress on seed germination and early seedling growth of wheat.DAE increased the seed germination rate and leaf water content of wheat under salt stress,enhanced the accumulation of biomass,the activity of superoxide dismutase,peroxidase,catalase,ascorbate peroxidase,as well as the content of proline,total soluble sugars,soluble proteins,and chlorophyll,but reduced lipid peroxidation,malondialdehyde content,and membrane permeability in leaves.Under 100 mmol/L NaCl,25 mg/L DAE had the best mitigating effect on wheat seed germination and early seedling growth.[Conclusion]By promoting the germination of wheat seeds,the Desmodesmus sp.extracts can improve the chlorophyll content,antioxidant enzyme activity,and osmotic adjustment ability of wheat seedlings to enhance the adaptability of wheat seeds and young seedlings to salt stress.

关 键 词：链带藻 盐胁迫 小麦 种子萌发 幼苗生长 生理指标 

分 类 号：Q945[生物学—植物学] S512[农业科学—作物学]