RcAGL61基因调控雄蕊和花瓣之间转变影响月季花瓣数量  

The RcAGL61 gene affects petal number by regulating the transition between stamens and petals in Rosa chinensis

作　　者：戚雨岑 杨淑敏 苏琳 杨艺 张启翔[1] 潘会堂[1] QI Yucen;YANG Shumin;SU Lin;YANG Yi;ZHANG Qixiang;PAN Huitang(Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding,National Engineering Research Center for Floriculture,College of Landscape Architecture,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,北京林业大学园林学院,北京100083

出　　处：《西北植物学报》2024年第6期904-911,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：北京市自然科学基金项目(6192018)。

摘　　要：[目的]为探究月季重瓣性状的调控机制,研究克隆了前期筛选到的1个与花发育相关的AG同源基因RcAGL61,并对其功能进行分析。[方法]用荧光定量对该基因在‘窄叶藤本月季花’ב月月粉’杂交群体中重瓣株系和单瓣株系花芽5个发育时期的表达模式进行分析,以重瓣株系和单瓣株系为材料,克隆RcAGL61,并进行生物信息学分析、亚细胞定位及VIGS实验。[结果](1)该基因表达水平在单瓣株系的5个发育时期均显著高于重瓣株系,在单瓣株系花发育的S4-S5期比S1-S3期表达量明显升高。(2)RcAGL61编码区序列在单瓣株系和重瓣株系中一致,长度为495 bp,与RcAG基因序列相似度为30.75%,编码164个氨基酸,含有1个MADS-box保守结构域,属于MADS-Box基因家族。(3)RcAGL61蛋白定位于烟草表皮细胞的细胞核。(4)沉默该基因后,瓣化雄蕊数量增加,雄蕊数量减少,花瓣数量增加,萼片数量和雌蕊数量无显著变化。[结论]RcAGL61参与调控雄蕊原基和花瓣原基间的转变,影响月季的花瓣数量。[Objective]The study aims to elucidate the regulatory mechanism of the double petal trait in rose.We cloned an AG homologous gene RcAGL61,which was previously screened and related to flower development,and examined its function.[Methods]qRT-PCR was used to analyze the expression of RcAGL61 in flower buds of the double-petaled lines and single-petaled lines in Rosa chinensis‘Zhaiye Tengben Yuejihua’×R.chinensis‘Old Blush’population.RcAGL61 was cloned from the double-petaled lines and single-petal lines,and bioinformatics analysis,subcellular localization,and VIGS experiments were carried out.[Results](1)The expression of RcAGL61 was higher in the five developmental stages of the singlepetaled line than the double-petaled line,and the expression was higher in S4-S5 stages than in S1-S3 stages in the single-petaled line.(2)The coding sequence of RcAGL61 was the same in the double-petaled line and single-petaled line,with a length of 495 bp,which had a similarity of 30.75%with RcAG.RcAGL61 encoded 164 amino acids,which contained a conserved MADS-box,belonging to the MADS-box family.(3)RcAGL61 was localized in the nucleus.(4)When silenced by VIGS technique in R.chinensis‘Old Blush’,the petalized stamens and petal numbers were increased,while the stamen numbers were decreased.The number of sepals and pistils had no significant change.[Conclusion]RcAGL61 is involved in regulating the transition between stamens and petal primordiums,and affects the number of petals in R.chinensis.

关 键 词：月季 RcAGL61 重瓣性 雄蕊瓣化 

分 类 号：Q785[生物学—分子生物学] Q786[农业科学—观赏园艺] S685.12[农业科学—园艺学]