抗/感灰霉病砧木嫁接对番茄叶片内生细菌群落结构的影响  

Effects of grafted resistant/susceptible rootstock against gray mold on tomato leaf endophytic bacterial communities

作　　者：王江珂 龚小雅 吴凤芝 WANG Jiangke;GONG Xiaoya;WU Fengzhi(School of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,Harbin 150030,China)

机构地区：[1]东北农业大学园艺园林学院,哈尔滨150030 [2]农业农村部东北地区园艺作物生物学与种质创制重点实验室,哈尔滨150030

出　　处：《东北农业大学学报》2024年第3期21-31,共11页Journal of Northeast Agricultural University

基　　金：财政部、农业部:国家大宗蔬菜产业技术体系专项资助(CARS-23-B09)。

摘　　要：筛选抗/感番茄灰霉病砧木并嫁接,对接种灰霉病菌后番茄叶片内生细菌进行高通量测序分析,探究番茄叶片内生细菌群落结构与组成差异。结果表明,在未受灰葡萄孢菌(Botrytis cinerea)胁迫时,抗病砧木嫁接番茄相比感病砧木嫁接番茄显著增加叶片内生细菌α多样性及潜在有益菌属苍白杆菌属(Ochrobactrum)、芽孢杆菌属(Bacillus)和链霉菌属(Streptomyces)相对丰度。接种灰葡萄孢菌后,抗病砧木嫁接番茄相比感病砧木嫁接番茄显著增加叶片潜在有益菌属苍白杆菌属、鞘氨醇杆菌属(Sphingobacterium)相对丰度,分属于有益菌属的OTU41和OTU57相对丰度与灰霉病发病率和病情指数呈显著负相关。抗病和感病砧木嫁接番茄叶片内生细菌群落结构差异显著,抗病砧木嫁接番茄提高抗病性可能与叶片富集特定有益内生细菌有关。In this study,resistant/susceptible rootstocks against tomato gray mold were screened and grafted.The endophytic bacterial communities in tomato leaves were analyzed after inoculation with Botrytis cinerea by high-throughput sequencing analysis,and then explored differences in the structure and composition of the endophytic bacterial communities.Furthermore,the potential relationship between the differential microbial community and disease occurrence was analyzed.The results showed that grafted tomatoes with disease-resistant rootstocks significantly increased the alpha diversity of the endophytic bacteria in the leaves and the relative abundances of potentially beneficial genus such as Ochrobactrum,Bacillus,and Streptomyces compared to grafted tomatoes with susceptible rootstocks under the absence of Botrytis cinerea.After inoculation with Botrytis cinerea,grafted tomatoes with disease-resistant rootstocks showed a significant increase in the relative abundances of potentially beneficial bacterial genus Ochrobactrum and Sphingobacterium in the leaves compared to grafted tomatoes with susceptible rootstocks.Additionally,the relative abundances of OTU41 and OTU57,belonging to bacterial genus Ochrobactrum and Sphingobacterium,respectively were significantly negatively correlated with the incidence and disease index of gray mold.There were significant differences in the structure of the endophytic bacterial communities in the leaves between tomatoes grafted with resistant and susceptible rootstocks.The improvement of disease resistance in tomato grafted with disease-resistant rootstocks might be related to the enrichment of specific endophytic beneficial bacteria in the leaves.

关 键 词：不同抗性砧木 嫁接 番茄 叶片内生细菌 灰霉病 细菌群落结构 

分 类 号：S641.2[农业科学—蔬菜学]