羊源溶血性曼氏杆菌SYS-1株分离鉴定及其毒力与耐药性分析  

Isolation,identification,virulenceand drug resistance analysis of SYS-1 strain of sheep-derived Mannheimia haemolytica

作　　者：刘芳[1] 苗苗 乌云那日苏 乌志勇 白伟琴 王金玲[1] 格日勒图[1] LIU Fang;MIAO Miao;Wuyunnarisu;WU Zhiyong;BAI Weiqin;WANG Jinling;Geriletu(School of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Xilingol League Agriculture and Animal Husbandry Bureau Comprehensive Support Center,Xilinhot Inner Mongolia 026000,China)

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010018 [2]锡林郭勒盟农牧局综合保障中心,内蒙古锡林浩特026000

出　　处：《东北农业大学学报》2024年第3期32-40,共9页Journal of Northeast Agricultural University

基　　金：内蒙古自治区自然科学基金项目(2021MS03047)。

摘　　要：绵羊上呼吸道疾病是兽医临床常见病,常见病因为溶血性曼氏杆菌感染。该菌具有多种血清型且呈区域性流行,造成当地绵羊养殖业严重经济损失。为探究陕西榆林羊场呼吸道疾病发病原因和危害,丰富该病防治措施,试验采集羊场有呼吸道病史的病死羊肺组织进行平板划线,对其分离的菌株进行形态学观察、生化试验及分子生物学鉴定及药敏性和小鼠致病性试验。结果表明,该菌株为A1型溶血性曼氏杆菌SYS-1株,含有毒力因子抗白细胞毒素,具有致死性风险。分离菌可发酵甘露醇和山梨醇,无法发酵甘露糖。吲哚试验结果阴性,过氧化氢试验呈阳性。该菌株生长缓慢,可对恩诺沙星、氟苯尼考等药物敏感,对庆大霉素和红霉素耐药。The upper respiratory tract disease of sheep is a common disease in veterinary clinical practice,and its etiology is more common due to the infection of Mannheimia haemolytica.This bacterium has multiple serotypes and is regionally prevalent,soit can cause serious economic losses in the local sheep breeding industry.This experiment collected dead sheep lung tissues with a history of respiratory diseases from the farm in Yulin,Shaanxi to explore the causes and hazards of the disease,and enrich the prevention and control measures of the disease.Morphological,biochemical,molecular biological identification,drug sensitivity testing,and mouse pathogenicity testing were performed on the isolated strains.The results showed that the isolated strain was the Mannheimia haemolytica strain SYS-1,serotype A1,with a virulence factor of LKT,which carried a lethal risk.This strain could ferment mannitol and sorbitol,but could not ferment mannose.The indole test result was negative,and the hydrogen peroxide test was positive.This strain showed slow growth,and was sensitive to drugs such as enrofloxacin and fluphenicol,and was resistant to gentamicin and erythromycin.

关 键 词：绵羊 溶血性曼氏杆菌 遗传进化树 毒力因子 

分 类 号：S852.65[农业科学—基础兽医学]