利用CRISPR-Cas9技术编辑ZmSh2基因创制超甜玉米新种质  

Using CRISPR-Cas9 Technique to Edit ZmSh2 Gene to Create New Germplasm of Super Sweet Maize

作　　者：吕庆雪[1] 汤继超[2] 周德龙 周迎鑫 吴广宇 孙蕾[1] 宋广树[1] LU Qing-xue;TANG Ji-chao;ZHOU De-long;ZHOU Ying-xin;WU Guang-yu;SUN Lei;SONG Guang-shu(Jilin Academy of Agricultural Sciences,Changchun 130033;Jilin Hongde Seed Industry Co.,Ltd.,Gongzhuling 136100;Gongzhuling Weizigou Street Integrated Service Center Agricultural Machinery Division,Gongzhuling 136106,China)

机构地区：[1]吉林省农业科学院,长春130033 [2]吉林吉农高新技术发展股份有限公司,吉林公主岭136100 [3]公主岭市苇子沟街道综合服务中心农机科,吉林公主岭136106

出　　处：《玉米科学》2024年第3期62-66,共5页Journal of Maize Sciences

基　　金：吉林省农业科技创新工程国际科技合作项目“玉米子粒品质改良种质材料创制研究”(CXGC202104GH)。

摘　　要：选取玉米ZmSh2基因,利用CRISPR-Cas9技术构建基因编辑载体pBUE411-ZmSh2,以玉米C01未成熟的幼胚组织为受体,利用农杆菌介导法将目的基因转入到玉米幼胚中,以草铵膦为选择剂进行筛选获得ZmSh2基因编辑的突变体株系。通过表型观察发现,编辑的纯合突变体材料明显比对照玉米自交系C01褶皱,使用手持式糖度仪检测突变体植株的甜度可达到25%。结果表明,利用CRISPR-Cas9技术能够创制出超甜玉米新种质材料,为CRISPR-Cas9介导玉米其他性状定向遗传改良提供了重要的理论和实践依据。The ZmSh2 gene of maize was selected and CRISPR-Cas9 technology was used to construct the gene editing vector pBUE411-ZmSh2.Immature embryos of maize CO1 were used as receptors and the target gene was transferred into young embryos of maize by tumefacien-mediated method.Mutant lines with edited ZmSh2 gene were screened using phosphine oxalate as the selector.Phenotypic observation showed that the edited homozygous mutant material was significantly more wrinkled than the control maize inbred line CO1.The sweetness of the mutant plants measured by a handheld sugar meter reached 25%.The results showed that CRISPR-Cas9 technology can be used to create new germplasm materials of super sweet maize and provide an important theoretical and practical basis for CRISPR-Cas9 to mediate the directed genetic improvement of other traits of maize.

关 键 词：玉米 CRISPR-Cas9技术 ZmSh2基因 遗传转化 

分 类 号：S513.035.3[农业科学—作物学]