防己有效成分通过抑制氧化应激反应减轻大鼠蛛网膜下腔出血后早期脑损伤的研究  

Effective Component of Fangji Alleviating Early Brain Injury after Subarachnoid Hemorrhage in Rats by Inhibiting Oxidative Stress

作　　者：冯智丽 靳晓飞 高维娟 钱涛[1,3] FENG Zhi-li;JIN Xiao-fei;GAO Wei-juan;QIAN Tao(Chengde Medical University,Chengde,Hebei,067000,China;Hebei University of Chinese Medicine,Shijiazhuang,050091,China;Hebei General Hospital,Shijiazhuang,050061,China)

机构地区：[1]承德医学院,河北承德067000 [2]河北中医药大学,石家庄050091 [3]河北省人民医院,石家庄050061

出　　处：《河北中医药学报》2024年第3期1-5,16,共6页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：河北省自然科学基金项目:No.H2022423382;河北省中医药管理局科研计划项目:No.2021101。

摘　　要：目的:探讨中药防己有效成分—粉防己碱(tetrandrine,Tet)改善大鼠蛛网膜下腔出血(subarachnoid hemorrhage,SAH)后早期脑损伤(early brain injury,EBI)的氧化应激反应的作用机制。方法:36只雄性SD大鼠随机分为假手术组(Sham组)、模型组(Model组)、粉防己碱组(Tet组),颅内血管内穿刺法建立SAH模型,Tet组术后腹腔注射Tet(20 mg/kg)。测定各组SAH出血量评分、神经功能缺陷评分和脑含水量。尼氏染色和HE染色观察脑皮质区神经元的形态和数量,透射电镜观察脑组织线粒体结构变化。生化法检测活性氧(ROS)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的含量。结果:与Sham组相比,Model组镜下脑组织损伤加重,脑含水量、ROS和MDA水平升高,神经功能缺陷评分、SOD、GSH-Px水平降低(P<0.05);相较Model组,Tet组镜下脑组织损伤减轻,脑含水量、ROS和MDA水平降低,神经功能缺陷评分、SOD、GSH-Px水平升高(P<0.05)。结论:中药防己有效成分—Tet可以抑制氧化应激反应,对大鼠SAH后早期脑损伤发挥保护作用。Objective:To investigate the mechanism of the effective component of Chinese medicine Fangji(tetrandrine)in improving oxidative stress response in early brain injury(EBI)after subarachnoid hemorrhage(SAH)in rats.Methods:Thirty-six male SD rats were randomly divided into sham operation group(Sham group),model group(Model group),and tetrandrine group(Tet group).The SAH model was established using intracranial vascular puncture,and the Tet group received intraperitoneal injection of tetrandrine(20 mg/kg)after surgery.The SAH bleeding score,neurological deficit score,and brain water content were measured in each group.Nissl staining and HE staining were used to observe the morphology and quantity of neurons in the cerebral cortex.Transmission electron microscopy was used to observe the structural changes of mitochondria in the brain tissue.Biochemical methods were employed to detect the levels of reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px).Results:Compared with the Sham group,the Model group exhibited aggravated brain tissue damage under microscopic observation,increased brain water content,ROS,and MDA levels,and decreased neurological deficit scores,SOD,and GSH-Px levels(P<0.05).In contrast to the Model group,the Tet group showed reduced brain tissue damage under microscopic observation,decreased brain water content,ROS,and MDA levels,and increased neurological deficit scores,SOD,and GSH-Px levels(P<0.05).Conclusion:The effective component of Chinese medicine Fangji can inhibit oxidative stress response and exert protective effects on early brain injury after SAH in rats.

关 键 词：防己 粉防己碱 氧化应激 早期脑损伤 蛛网膜下腔出血 

分 类 号：R285.5[医药卫生—中药学]