富血小板血浆-80℃深低温短期保存后细胞因子的变化  

Changes in cytokines in platelet-rich plasma after short-term cryopreservation at-80℃

作　　者：蔺锡桐 刘加秀[1,2] 张树超[1,2] LIN Xitong;LIU Jiaxiu;ZHANG Shuchao(Department of Immunology,School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院免疫学系,山东青岛266071 [2]青岛大学附属医院输血科

出　　处：《精准医学杂志》2024年第3期232-235,共4页Journal of Precision Medicine

基　　金：国家自然科学基金青年项目(81502246)。

摘　　要：目的分析富血小板血浆(PRP)-80℃深低温短期保存以后,其内P-选择素、血管内皮生长因子(VEGF)、血小板源生长因子(PDGF)浓度及其pH值等重要指标的变化,为PRP低温储存提供理论依据。方法取30例健康供者血液制备的PRP,每份样本分装成5份,每份留取0.5 mL,分别为A组(新鲜组)和B~E组(分别冻存5、10、15、20 d)。测定各组PRP的pH值;ELISA法测定各组PRP中P-选择素、PDGF和VEGF的水平;血管形成实验检测A组和E组对HUVEC细胞血管形成能力的影响。结果各组pH值比较差异无显著性(P>0.05);各组PRP中P-选择素、PDGF、VEGF的浓度比较,差异均无显著性(P>0.05);A组和E组中HUVEC血管形成数量比较差异无显著意义(P>0.05)。结论-80℃深低温保存PRP对其pH值、P-选择素、VEGF和PDGF浓度变化无显著影响,可延长PRP临床应用时间。Objective To analyze the changes in important indicators such as P-selectin,vascular endothelial growth factor(VEGF),platelet-derived growth factor(PDGF),and pH value in platelet-rich plasma(PRP)after short-term cryopreservation at-80℃,and to provide a theoretical basis for the low-temperature storage of PRP.Methods Each of PRP samples prepared from the blood from 30 healthy donors was divided into 5 aliquots,with 0.5 mL retained per aliquot.These samples were de-signated as group A(fresh sample)and groups B to E(cryopreservation for 5,10,15,and 20 d,respectively).The pH values of the five groups were measured.The concentrations of P-selectin,PDGF,and VEGF in each group were determined by the ELISA method.The effect of groups A and E on the vascular formation capacity of human umbilical vein endothelial cells(HUVEC)was determined by the angiogenesis assay.Results No significant differences were observed in the concentrations of P-selectin,PDGF,and VEGF as well as the pH values between group A and groups B to E(P>0.05).Compared with the fresh sample(group A),the number of vessels formed from HUVECs in the sample frozen for 20 d(group E)was basically the same,with no significant differences(P>0.05).Conclusion The cryopreservation of PRP at-80℃has no significant effects on its pH value and concentrations of P-selectin,VEGF,and PDGF,thus extending the clinical application time of PRP.

关 键 词：富血小板血浆 低温保存 P选择素 血管内皮生长因子类 血小板源性生长因子 氢离子浓度 

分 类 号：R457.14[医药卫生—治疗学] R318.52[医药卫生—临床医学]